Plenary Talks

Abstract

The Charnley hip implant revolutionized medicine returning motor function to millions. Over the decades since the Charnley hip implant was first introduced to medicine, numerous researchers have tried to improve the functionality of hip implants, from changing chemistry, geometry, surface texture, and even using injectable chemistries. This talk will summarize some of the more promising advances, in particular what has been seen with nanotechnology (or the use of materials with at least one dimension less than 100nm). Specifically, increased bone formation, decreased infection, and reduced inflammation have all been observed by employing nanoscale surface features (and without drugs) on the traditional Charnley implant regardless of chemistry. This talk will cover such results emphasizing those which have received FDA approval and are currently helping hundreds of patients return to an active lifestyle. Moreover, this talk will discuss the fundamental reasons why nanotechnology is so promising in orthopedic medical device applications.

Biography

Thomas J. Webster’s (H index: 88) degrees are in chemical engineering from the University of Pittsburgh (B.S., 1995) and in biomedical engineering from Rensselaer Polytechnic Institute (M.S., 1997; Ph.D., 2000). Prof. Webster has graduated/supervised over 149 visiting faculty, clinical fellows, post-doctoral students, and thesis completing B.S., M.S., and Ph.D. students. To date, his lab group has generated over 13 textbooks, 68 book chapters, 376 invited presentations, at least 503 peer-reviewed literature articles and/or conference proceedings, at least 767 conference presentations, and 42 provisional or full patents. He is the founding editor-in-chief of the International Journal of Nanomedicine (pioneering the open-access format). Prof. Webster currently directs or co-directs several centers in the area of biomaterials: The Center for Natural and Tropical Biomaterials (Medellin, Colombia), The Center for Pico and Nanomedicine (Wenzhou China), and The International Materials Research Center (Soochow, China). He has received numerous honors and is current a fellow of AANM, AIMBE, BMES, IJN, NAI, RSM, and FSBE.

Speaker
Thomas J. Webster / Northeastern University, USA

Abstract

The development of biologically inspired materials typically involves extensive trial-and-error studies. Rational understanding and design using modeling and simulation become increasingly feasible due to more accurate models and affordable computing resources. We will share atomic-level insights insights into biomaterials properties at the 1 to 1000 nm scale using the Interface force field (IFF), including recognition and assembly of metal, oxide, and biomineral nanostructures mediated by biomolecules and polymers. Examples include nucleation and growth of bone, low dimensional materials, catalysts, hydrogels, and therapeutics. We will then discuss new opportunities using reactive simulations (IFF-R) and data science tools to learn and interpret the information contained in large computational and experimental data sets to accelerate property predictions. We outline the process of generating a feature representation, the translation into reinforcement learning with nodes and edges, and Bayesian-based uncertainty quantification of predicted properties. Requirements for data sets and first applications will be described.

Biography

Hendrik Heinz received his Ph.D. degree from ETH Zurich, carried out postdoctoral work at the Air Force Research Laboratory, is an Associate Professor at the University of Colorado at Boulder and a Fellow of the Royal Society of Chemistry. His research focuses on the simulation of biomaterials and nanomaterials from the nanoscale to the microscale. He leads the development of the Interface force field and surface models for the simulation of compounds across the periodic table in high accuracy, including minerals, aloys, 2D materials, proteins, polymers. He received Special Creativity and Careers Awards from NSF, the Sandmeyer Award from the Swiss Chemical Society, and the Max Hey Medal from the Mineralogical Society.

Speaker
Hendrik Heinz / University of Colorado, USA

Abstract

Chirality is one of life’s most distinctive biochemical signatures and has great influence on many biological events, e.g. maintaining normal functions for living cells. It reveals that cells can sense surface chiral molecules to show differential behaviors on enantiomorphous surfaces. So far, the researches are mainly confined to the role of molecular chirality on two dimensional (2D) surface and a lot of questions remaining to be answered. Among them, how nanofibrous chirality influences cell behaviors in three dimensional (3D) extracellular matrix (ECM) is especially important, since it is only the 3D ECM nanofibrous structure can really mimick the necessary biophysical environment for tissue engineering and helical nanofibrous structure is closely related with the relevant biological events. To explore this, supramolecular gelators are of particular interest candidate because their assembly arises from non–covalent interactions. With the rational design of chemical composition and molecular structures, surpramolecular gelators can be efficiently self-assemble into two or three dimensional chiral microstructures, showing a big potential as biomimetic scaffold for multi-dimensional cell culture. With variation of physical or chemical properties, the chiral structures with the varied surface composition, mechanical strength, and surface wettability can be constructed and chirality regulated cell adhesion can be obtained in 3D. It is found that left-handed hydrogels can enhance cells adhesion and proliferation, but not for right-handed hydrogels. A smart control of cell adhesion is also realized by applying external fields, such as light, pH and so on. The study paves a way to explore the influence of chiraity of nanostructures on cell behaviors cell culture in 3D chiral environments and this chiral materials have potential application in the field of tissue engineering. For example, recently, stem cells are successfully cultured in 3D and it is found that stem cells can successfully differentiate into Osteoblast, however, stem cells are inhibited to differentiate.

Biography

Prof. Dr. Chuanliang Feng received Doctor Degree from University of Twente (the Netherlands) in 2005. After this, he was awarded Max-Planck Society Scholarship to work at Max-Planck Institute for Polymer Research in Mainz. From 1998 to July 2009, he was appointed as a research scientist in Biomade Technology Foundation (Groningen, the Netherlands). In Aug. 2009, he moved to Shanghai Jiaotong University as a full professor in School of Material Sciences and Technology. He was supported by Program for New Century Excellent Talents in University, Program for Shanghai Pujiang Excellent Talents, and Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning. His research mainly focuses on Chiral biomaterials, polymeric materials, supramolecular hydrogels. Important topics are synthesis and characterization of chiral supramolecular hydrogels and biomimetic materials as well as applications of biomaterials in Regenerative Medicine. He has published more than 110 papers, including J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater. and so on. He has more than 20 patents. He is guest editor of European Polymer Journal, and editorial member of Advance Hybrid and Composite Materials. Recently, he has been awarded Richard Robert Ernst during PolyChar 2019 international conference due to his innovation contribution on supramolecular chemistry.

Speaker
Chuanliang Feng / Shanghai Jiao Tong University, China

Keynote Talks

Abstract

Among adult mesenchymal stem cells, adipose-derived stem cells (ADSCs) hold great promise in tissue engineering and cell therapy. Adult mesenchymal stem cells can overcome the legal and ethical issues associated with the use of embryonic stem cells, and the risk of tumorigenicity of induced pluripotent stem cells. In addition, these cells can be used in autologous form. ADSCs are advantageous because they can be obtained in a relatively large quantities and by a less invasive method, i.e. from subcutaneous fat obtained by liposuction. These cells have the capacity to differentiate into various cell types, which can be facilitated by suitable mechanical stimulation, coupled with appropriate composition of the cell culture media. In our experiments, the differentiation of ADSCs towards vascular smooth muscle cells has been achieved by pulse pressure stimulation in a custom-designed bioreactor, or by uniaxial stretching in the commercially available STREX system, combined with the presence of TGF-beta1 and BMP-4 in the culture medium [1, 2]. ADSCs also differentiated relatively easily towards osteoblasts, which was facilitated by vibrational stress in combination with osteogenic media containing dexamethasone, β-glycerol phosphate, ascorbic acid, and in some cases also L-glutamine and dihydroxyvitamin D3 [1-3]. The differentiation of ADSCs towards cells with polarity, i.e. with functional specialization of the basal and apical cell membrane, such as vascular endothelial cells and keratinocytes, is considered to be difficult, but it can be facilitated by laminar shear stress in the case of endothelial cells, and by uniaxial strain or by pressure stress in the case of keratinocytes [1]. This review lecture summarizes recent knowledge on differentiation of ADSCs towards various cell types, supported by appropriate dynamic loading.

Biography

Lucie Bacakova, MD, PhD, Assoc. Prof. has graduated from the Faculty of General Medicine, Charles University, Prague, Czechoslovakia in 1984. She has completed her Ph.D at the age of 32 years from the Czechoslovak Academy of Sciences, and became Associated Professor at the 2nd Medical Faculty, Charles University. She is the Head of the Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic. She is a specialist for studies on cell-material interaction and vascular, bone and skin tissue engineering. She has published more than 150 papers in reputed journals.

Speaker
Lucie Bacakova / Institute of Physiology of the Czech Academy of Sciences, Czech Republic

Abstract

Direct metal laser sintering (DMLS) is a new additive manufacturing technique that allows solids with complex geometry to be fabricated by annealing metal powder microparticles in a focused laser beam, according to a computer-generated three-dimensional model. The fabrication process involves the laser-induced fusion of metal microparticles, in order to build, layer by layer, the desired object. With DMLS, it is possible to fabricate titanium implants with an inherently porous surface, a key property required of implantation devices. Human histologic/histomorphometric studies have demonstrated the potential of DMLS implants to osseointegrate in human bone under different loading conditions. Clinical studies have confirmed a high success rate for DMLS titanium implants used in different clinical contexts, and under different protocols. Finally, with DMLS, patient-specific implants (root-analogues, blade and maxillofacial implants) can be produced, adapting the implant to the anatomy of the patient instead of adapting the patient�s bone to a preformed standardized fixture.

Biography

Dr. Francesco Mangano was born in 1979 on the Lake of Como, Italy. He graduated at the University of Milan in 2003 (110/110 cum laude). Immediately after graduation, he devoted his attention to Implant Dentistry, attending the Post-graduate Program in Oral Implantology and Bone Regeneration of the Oral Implantology Clinic, Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo (Brazil), where he became Clinical Instructor. After this experience, he worked intensively in the field of Implant Prosthodontics and Digital Dentistry. He is Lecturer for the Academic Unit of Digital Dentistry, IRCCS, San Raffaele Hospital, Milan, (Italy), Founder and Scientific Coordinator of the first-in-the-world 2-year Master in Digital Dentistry, at the University of Varese (Italy). In 2016, he completed his PhD in Biotechnologies, Biosciences and Surgical Techniques, at the Department of Surgical and Morphological Sciences, University of Varese (Italy). From 2018, he is Associate Professor and Lecturer at the Sechenov First State Medical University of Moscow, Russia. He is Section Editor for the Digital Dentistry Section of BMC Oral Health, Associate Editor for Journal of Dental Research, Dental Clinics, Dental Prospects, and Lead Guest Editor of International Journal of Dentistry, Biomedical Research International (Tissue Engineering Section) and The Open Dentistry Journal; in addition, he works as Reviewer for the most important international, peer-reviewed journals with high impact factor. He is Fellow of the International College of Dentists, and Founding and Board Member of the international Digital Dentistry Society (DDS). He is author of 100 scientific publications indexed in Pubmed, and published on international peer-reviewed Journals with high impact factor. His bibliometric indexes are: impact factor 177.5 (Researchgate), H index 31 (Google Scholar). He co-authored three books on implant dentistry and guided implant surgery. He is in private practice in Gravedona, Como, Italy, together with Prof. Carlo Mangano, his uncle and mentor.

Speaker
Francesco Guido Mangano / Sechenov First State Medical University of Moscow,Russia

Abstract

The fabrication of nanoporous / nanoparticulate composites and their applications via surface patterning with chemicals and bio-chemicals has a direct impact in bio-sensing and bio-separation. Surface patterning on nanoparticles in suspension can be a complex process due to the aggregation of the particles and their Brownian motion in the suspension. An overview of group�s research on nanomaterials and their applications in the separation of nucleic acids (DNA and RNA) from the biological cells will be presented in connection with an industrial collaboration with Q-Bioanalytic, Germany. The possibility of affinity interaction of biomolecules i.e. nucleic acid, protein, antibody, microorganisms etc. through hybrid capture will also be discussed in the context of food quality and hygiene in Bio-sensing which has recently been published in Nature publishing group (http://www.nature.com/srep/2012/120807/srep00564/full/srep00564.html?WT.ec_id=SREP-639-20120903). Separation of toxic and microbial contaminants from water and soil using nanotechnology tool will be discussed in the context of on-going multinational projects (http://senlabs.org/international-projects/ & http://nanowateratuclan.org/) in collaboration with top academic and industrial researchers from Europe, India and China. Recent development (UK Patent: 2013: GB1315407.5. & PCT/GB2014/052,630) on sensing antimicrobial nanocomposites will be discussed in connection with water technology.

Biography

Dr. Tapas Sen completed his first degree in Chemistry (BSc. Hons) followed by a Masters in Physical Chemistry (MSc) and a PhD in Materials Chemistry from the premier research institution, National Chemical Laboratory, Pune, India. He worked at the Weizmann Institute of Science, Israel during 1997 to 1999 in the world leading Solid state NMR group as a post-doctoral visiting scientist. He then moved to UK in February 2000 and worked as a postdoctoral Research Fellow in the University of Manchester Institute of Science and Technology (UMIST), Manchester (2000-2003) on an industrial project funded by ICI Synetix. He later worked on multimillion Euro projects under EU framework V and VI programmes (2003-2008) in various parts of UK before joining as a lecturer in Chemistry (December 2008) at the University of Central Lancashire. Currently he is leading the Nano-biomaterials Research group dedicated on researching in the area of nanomaterials and their applications in separation science, drug delivery, industrial catalysis and bio-sensors. Currently the group is running three multinational projects in collaboration with world leaders from academia and industries.

Speaker
Tapas Sen / University of Central Lancashire, UK

Abstract

Recent advances and applications of biomolecule-responsive hydrogels in medicine via emphasizing this research area with novel biomaterials technology have shown great interest in medical applications. Protein-responsive hydrogels are classified into two major types, including enzyme-responsive hydrogels and antigen-responsive hydrogels. In the present study, we willdiscuss the recent applications of nucleic acid-responsive hydrogels based on four main categories: RNA-responsive hydrogels, DNA-responsive hydrogels, aptamer-responsive hydrogels and PNA-responsive hydrogels. We will further show the recent application of these modifiedhydrogelsindrug/gene delivery, diabetes, biosensor, tissue engineering, and cell and cancer area.

Biography

Dr. Hosseinkhani has broad experience in life sciences and is expert in nanotechnology, biomaterials, drug delivery, 3D in vitro systems, bioreactor technology, and bioengineering stem cells technology. He has long experience in both academia and industry in biomedical engineering research and development, which includes several years of basic science research experience in a number of premier institutions related to the structure and function of biomaterials, and in polymer-based and mineral-based medical implants development in the medical device industry.He has been awarded several prestigious fellowships including JSPS Fellowship of Japan, and European Marie Curie Fellowship. Dr. Hosseinkhani has several issued/pending U.S. patentsand has authored over 100 international publications in prestigious international journals and over 200 presentations at international conferences. He is the founder and chief science officer at Matrix, Inc. a world leading biotech company dedicated to healthcare technology to improve patient's quality of life.

Speaker
Hossein Hosseinkhani / Founder & Chief Science Officer, Matrix, Inc, USA

Abstract

Co-ordination assisted self-assembly of the macromolecules is one of the unique attributes to accomplish a stimuli-responsive monodispersednano-formulation (NF) applicable in biomedicines. The stimuli-responsive structural reorganization of these NF's draws anenormous attention in the field of NF based biotherapy. Herein we report two different types of smart-formulation showing stimuli-responsive structural articulation followed improvised in the specific biological environments. Firstly, the macromolecule, pentaerythritol poly(caprolactone)-b-poly(acrylic acid) form Fe+3 ion induced light-responsive NF with the unique structural arrangement as like spherically shaped human brain. The DOX (chemo therapeutic agent)-loaded NF undergoes structural deformation in the presence of light and shows a release of DOX molecule (85.2% at 120 min). Administration of the DOX-loaded NF to C6 glioma rat model (in vivo) offered tremendous inhibition (∼91%) of tumor growth without any toxic side effects. Secondly, mannose conjugated antimicrobial polypeptide, poly(arginine-r-valine)-mannose undergoes Zn2+ ion induced self-assembly into a NF with a unique structural appearance as like Taxus baccata fruits. The NF uptake by the bacterial membrane led structural deformation followed by exposing of free polypeptide molecules. These molecules are enforced to lysis the bacterial membrane followed by diffusion of cytoplasmic component out of the membrane that culminates final death of bacteria (MIC values varies from 0.67 to 2.55 µM). Indeed, NF’s remainnon-toxic against both the mammalian as well as red blood cell as reflected from their higher order of cell viability (˃ 80%) and very insignificant hemolytic effect (˂13%). Hence, metal ion assisted self-assembly approach brings about a new therapeutic window, where the fully exposed macromolecule can be formulated into compact NF with enhanced therapeutic performance.

Biography

Prof. (Dr.) Santanu Chattopadhyay is associated with Rubber Technology Centre, Indian Institute of Technology Kharagpur, WB, India for last 15 years. Currently he is Professor and Head of Rubber Technology Centre. He is also an adjunct Professor of School of Nano Science and Technology, IIT Kharagpur. Before that, he worked as postdoctoral research associate at Georgia Institute of Technology, Atlanta, GA, USA for two years and postdoctoral fellow at The University of Western Ontario, London, Ontario, Canada for two years. He completed his PhD in 2001 from Indian Institute of Technology Kharagpur, WB, India and M.Tech from Indian Institute of Technology, Mumbai, India. He also did Masters in Chemistry from Indian Institute of Technology Kharagpur, WB, India and Bachelors from University of Calcutta, Kolkata, India. His research interests cover the area of synthesis and characterisation of block copolymers, biomaterials, and polymers for health care as well as energy harvesting. He is also working on smart rubber composites and FEA of rubbery/textile materials. He has remarkable publications in the reputed international journals like Chemistry of Materials, ACS Applied Materials Interfaces, Biomaterials, Langmuir, Macromolecules, RSC Advances and others in the field of biomaterials along with publications in the field of polymers, rubbers and nanocomposites. He has more than 120 journal and 75 conference publications, four book chapters and two patents. He has guided 14 PhD students and 17 PhD are ongoing. Currently, he is also acting as one of the editorial board members of Journal of Advanced Biotechnology and Bioengineering and reviewing manuscripts for 19 international journals

Speaker
Santanu Chattopadhyay / Rubber Technology Center, India

Abstract

Pore-forming toxins (PFTs) are the most common bacterial virulence proteins and play a significant role in the pathogenesis of bacterial infections; thus, PFTs are an attractive therapeutic target in bacterial infections. Inspired by the pore-forming process and mechanism of PFTs, we designed two RBC-mimicking liposomes�the erythroliposome and the erythrosome�for PFT detoxification by fusing natural red blood cell (RBC) membranes or RBC membrane proteins with artificial lipid membranes. With α-hemolysin (Hlα) as a model PFT, we demonstrated that both the erythroliposome and the erythrosome could not only significantly reduce the toxicity of Hlα to erythrocytes in vitro but also effectively sponge Hlα in vivo and rescue mice from Hlα-induced damage. Consequently, the RBC-mimicking liposome nanoplatform inspires potential strategies for antivirulence therapy.

Biography

Ph.D Zhiqing Pang is an associate researcher of School of Pharmacy, Fudan University. He obtianed his Ph.D. in Pharmaceutics from Fudan University in 2008. His research mainly focuses on smart drug delivery systems especially in brain drug delivery. He published >100 peer-review scientific papers, with citation by >4,500 times (H-index~39); coedited 4 books in English and 3 books in Chinese. His scientific achievements have been honored by some scientific awards in China, including National Award for Science and Technology (2018); Distinguished Young Scholar of Chinese Pharmaceutical Association-Sanofi (2017), Natural Science Award of Ministry of Education (2010).

Speaker
Zhiqing Pang / Fudan University, China

Sessions:

Abstract

Superelastic NiTi alloy is the current primer material used in an extensive variety of biomedical and dental applications, mainly due to its exceptional superelasticity. However, the toxicity of Ni and the large Ni content of NiTi (~51 at.%) are concerning. Hence, a large number of Ni-free biocompatible superelastic alloys have been developed in recent years, out of which only a few exhibit superelasticity that is comparable with that (~7%) of NiTi. The large superelasticity exhibited by these alloys is attributed to a strong recrystallization texture induced by severe mechanical processing. Here we present a novel class of Sn-containing solute-lean Ti alloys which offer excellent superelasticity (~5%) without a crystallographic texture. The influence of Sn content on superelasticity, mechanical properties and deformation mechanisms in these alloys will be discussed. Also discussed are the superior mammalian cell response and corrosion resistance of these alloys compared with NiTi. These considerations indicate the potential of these novel alloys for biomedical cardiovascular, bone-replacement and dental implant applications.

Biography

Mr Ali Ramezannejad is a current PhD student at the Royal Melbourne Institute of Technology (RMIT University). His PhD research is focused on design and development of new biomedical nickel-free superelastic titanium alloys with superior biocompatibility and corrosion resistance compared with that of the NiTi alloy. He has two recent publications regarding this topic.

Speaker
Ali Ramezannejad / RMIT University, Australia

Abstract

Introduction: Acid-base equilibrium is one of the most important factors that influence behaviors of bone cells. In scenario of osteoporotic fracture, significantly higher activity of osteoclasts than osteoblasts may lead to continuous loss of bone in fracture/defect site. In that case, we propose modulating the microenvironment pH (μe-pH) of that milieu, which is influenced by implants surface chemistry and biodegradation, to re-establish normal bone regeneration at the fracture site. Methods: In our series studies, the measurement of material interfacial pH was realized by using the pH microelectrode. In vitro and in vivo examinations were conducted for evaluating the pH’s effect on defect regeneration process in both normal/pathological conditions. Results: We demonstrated that the pH at the material surface is different from that of a homogeneous bulk extract at an early stage in vitro, and the altered local pH affects significantly the proliferation and ALP activity of MG-63 osteoblast-like cells. We have further revealed that the weakly-alkaline surface pH (pH 8–8.5) showed most stimulated effect on MC-3T3 cells viability and activity, thus further facilitating apatite nucleation. The increased culture environment pH has also been proved to stimulate osteogenic differentiation of osteoporosis bone marrow stromal cells (pH 7.73-7.94), and suppress the differentiation and pit-formation activity of RAW 264.7 osteoclast precursors (pH 7.59-8.02). In vivo, alkaline biodegradable materials generated a μe-pH which was higher than the normal physiological value, in particular, at the initial stage. Higher μe-pH is associated with better overall performances: greater new bone formation, suppression the activity of TRAP+ osteoclast-like cells, as well as the formation of an intermediate ‘apatitic’ layer. The osteoclastic associated enzymes have been proved to be greatly involved in the pH-related suppression effect towards osteoclasts.

Biography

Prof. Haobo Pan completed his PhD studies at the University of Hong Kong in 2007, and carried out his postdoctoral research at the Li Ka Shing Faculty of Medicine in the University of Hong Kong from 2008 to 2010. In 2010, he promoted to Research Assistant Professor at the Department of Orthopaedics and Traumatology in the University of Hong Kong. In 2012, he was appointed as the Director of the Research Center in Human Tissues and organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science. Later, he was promoted as the Vice Director of the Institute of Biomedicine and Biotechnology and appointed as the Director of Shenzhen Key laboratory in Marine Biomaterials and the Director of Technology Center of Guangdong Province Marine Biological Materials Engineering. His current research activities are focused on material chemistry, biomaterial synthesis and modification, marine biotechnology, biomaterial zoology and clinical assessment. He has published more than 100 papers in qualified international journals and served as board member of ISRN Biomaterials and Journal of Osteoporosis and Physical Activity.

Speaker
Haobo Pan / Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, China

Abstract

Recently, sorafenib being studied in combination therapy in colorectal cancer (CRC) attracted attention of researchers. On the basis of our previous study, pigment epithelium-derived factor (PEDF) loaded nanoparticles showed good effect on CRC in vitro and in vivo. Herein, we designed a combination therapy for sorafenib (Sora), a multi-kinase inhibitor and PEDF, a powerful antiangiogenic gene, in a nano-formulation aimed to increase anti-tumor effect on CRC for the first time. Sora and PEDF gene were simultaneously encapsulated in PEG-PLGA nanoparticles by a modified double-emulsion solvent evaporation method. The obtained co-encapsulated nanoparticles (Sora@PEDF-NPs) showed high entrapment efficiency of both Sora and PEDF gene and exhibited a uniform spherical morphology. The release profiles of Sora and PEDF gene were in a sustained manner. The most effective tumor growth inhibition in the C26 cells and C26-bearing mice was observed in the Sora@PEDF-NPs in comparison with none-drug nanoparticles, free Sora, mono-drug nanoparticles (Sora-NPs and PEDF-NPs) and the mixture of Sora-NPs and equivalent PEDF-NPs (Mix-NPs). More importantly, Sora@PEDF-NPs showed lower toxicity than free Sora in mice according to the acute toxicity test. The serologic biochemical analysis and mice body weight during therapeutic period revealed that Sora@PEDF-NPs had no obvious toxicity. All the data demonstrated that the simultaneously loaded nanoparticels with multi-kinase inhibitor and antiangiogenic gene might be one of the most potential formulations in the treatment of colorectal carcinoma in clinic and worthy of further investigation.

Biography

Hongtao Xiao has completed completed his doctorate in biomedical engineering with the theoretical and practical combination of pharmacy and pharmacology at the Tongji Medical University, China, West China School of Pharmacy Sichuan University,University of Electronic Science and Technology of China for bachelor, master, PHD, respectively. After his pharmacogenetics study in University of California San Francisco, he joined the Sichuan Cancer Hospital&Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China as the dean and professor. Now he is focused on cancer pharmacology and gene therapy of cancer, he published about 80 papers and 7 patens. He also got a lot of grant from government.

Speaker
Hongtao Xiao / University of Electronic Science and Technology of China, China

Abstract

A folic acid (FA) functional drug delivery system (MT@LPTX@FA) based on in situ formation of tellurium nanodots (Te NDs) in paclitaxel (PTX)-loaded MgAl layered double hydroxide (LDHs) gated mesoporous silica nanoparticles (MSNs) has been designed and fabricated for targeted chemo/PDT/PTT trimode combinatorial therapy. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), N2 adsorption-desorption, Fourier transform infrared (FT-IR) spectra, and UV-vis spectra were used to demonstrate the successful fabrication of MT@L-PTX@FA. In particular, the in situ generated Te NDs showed a homogeneous ultrasmall size. Reactive oxygen species (ROS) generation, photothermal effects, and photostability evaluations indicated that the in situ generated homogeneous Te NDs could serve as the phototherapeutic agent, converting the photon energy to ROS and heat under nearinfrared (NIR) irradiation efficiently. The drug-release test revealed that MT@L-PTX@FA showed an apparent sustained release character in a pH-sensitive manner. In addition, cell imaging experiments demonstrated that MT@L-PTX@FA could selectively enter into cancer cells owing to the function of FA and release of PTX efficiently for chemotherapy for the reason that the low intracellular pH would dissolve MgAl LDHs to Mg2+ and Al3+. Cytotoxicity tests also indicated that MT@L-PTX@FA exhibited enhanced therapeutic effect in cancer cells under NIR irradiation, benefiting from the synergy based on targeted chemo/PDT/PTT trimode combinatorial therapy. The preliminary results reported here will shed new light on the future design and applications of nanosystems for synergistic combinatorial therapy.

Biography

Shiguo Sun has completed his PhD from Dalian University of Technology, China and postdoctoral studies from Royal Institute of Technology, Sweden. He is a professor and doctoral supervisor at the College of Chemistry & Pharmacy, Northwest A&F University, China. He has published more than 150 papers in reputed journals. His research interests include Systematic Targeting Pharmaceutics (STP); Visible Sensor Guided Drug Delivery and Targeting; Fluorescent Probe and Imaging; Visible Detection of Organelle, Tumor and Virus etc.

Speaker
Shiguo Sun / College of Chemistry & Pharmacy, Northwest A&F University, China

Abstract

Statement of the Problem: Women who have experienced intimate partnerviolence(IPV)areatgreaterriskforphysicalandmentalhealth problems including posttraumatic stress disorder (PTSD) and alcohol dependency. On their own IPV, PTSD and alcohol dependency result in significant personal, social and economic cost and the impact of all threemaycompoundthesecosts.Researchershavereportedthatwomen with these experiences are more difficult to treat; many donotaccess treatmentandthosewhodo,frequentlydonotstaybecauseofdifficulty maintaining helping relationships. However, these women�s perspective has not been previously studied. The purpose of this study is todescribe the experience of seeking help for alcohol dependency by women with PTSD and a history of IPV in the context in which it occurs. Methodology & Theoretical Orientation: An intersubjective ethnographic study using hermeneutic dialogue was utilized during participant observation, in- depth interviews and focus groups. An ecological framework was utilizedtofocusontheinteractionbetweenthecounselorsandthestaffto understand this relationships and the context in which it occurs. Findings: Thewomeninthisstudywereveryactivehelpseekers.Theyencountered many gaps in continuity of care including discharge because ofrelapse. Although the treatment center was a warm, healing and spiritual place, the women left the center without treatment for their trauma needs and many without any referral to address these outstanding issues. Conclusion & Significance: Women with alcohol dependence and PTSD with a history of IPV want help however the health and social services donotalwaysrecognizetheircallsforhelportheirsymptomsofdistress. Recommendations are made for treatment centers to become trauma- informed that would help thisrecognition.

Biography

Mahdi Hadi has completed his higher educationat the age of 30 years fromIslamic Azad UniversityØŒScience And Research Branch in Iran. He is Director of Regenerative medicine and Head of Advanced Biological Technologies Departmentat atTofigh Daru Research & Engineering Company in Tehran and is engaged in developing advanced bio-based pharmaceuticals complying with GMP grade conditions. He has been involved in stem cell and tissue engineering in research and industrial centers of Iran for 12 years. He has worked as a Researcher and Head of Embryo biotechnology Department at Royan Institute, Tehran for 8 Year. He has worked as the Project Manager of Cell Therapy Medicines Production and Tissue Engineering in pharmaceutical company.He has more than 50 papers, abstracts, lectures and book. He was a keynote speaker at Advanced Biomaterials and Tissue Engineering 2018 in Rome, Italy

Speaker
Mahdi Hadi / Tofigh Daru Research & Engineering Company, Tehran, Iran

Abstract

Innovative strategies for periodontal regeneration have been the focus of research clusters across the globe for decades. In order to overcome the drawbacks of currently available options investigators have suggested a concept of functionally graded membrane (FGM) with different structural and morphological gradients. Chitosan have been used in the past for similar purpose. However, the composite formulation of composite and tetracycline when cross-linked with glutaraldehyde have received little attention. Therefore, the purpose of the study was to investigate the drug loading and release characteristics of freeze casted chitosan scaffolds at different percentages of glutaraldehyde. Freeze casted chitosan templates were prepared at concentrations of 2, 4 and 6%. These were crosslinked with 0.1 and 1% glutaraldehyde and loaded with doxycycline hyclate. Prepared composite templates were charactized with electron micrographs, computerized tomography, Fourier transform infrared spectroscopy, swelling analysis, drug loading and release profile over a period of 24 hrs. The electron micrographs depicted porous morphology of neat templates. After cross-linking these templates showed compressed ultrastructures. Computerized tomography analysis showed that the templates had 88 to 92% porosity with average pore diameter decreased from 78 to 44.9 �m with increasing concentration. Fourier transform infrared spectroscopy showed alterations in the glycosidic segment of chitosan finger print region which after drug loading showed a dominant doxycycline spectral composite profile. Interestingly swelling profile was not effected by cross-linking either at 0.1 and 1 % glutaraldehyde and template showed a swelling ratio of 80 %, which gained equilibrium after 15 minutes. The drug release pattern also showed a 40�g /ml of release after 24 hours. These doxycycline-loaded templates show their tendency to be used in a functionally graded membrane facing the defect site

Biography

Saad graduated with a bachelors degree in dentistry from Baqai Medical University, Karachi, Pakistan in 2005, he received a Masters degree in Dental Materials from Queen Mary University of London in 2008. After his MSc he worked as clinician and an academic before joining The King Saud University in Riyadh, Saudi Arabia as a researcher at the dental biomaterials research chair, in july 2012 he moved to Sheffield, United Kingdom. There he received his doctorate in biomaterials science from The University of Sheffield with a PhD thesis entitled � Development of a novel functionally graded Guided tissue regenerative membrane for periodontal lesions� in 2015. Saad is also currently involved in ongoing research at the Kroto Research Institute, University of Sheffield.

Speaker
Syed Saad B Qasim / University of Oslo, Norway

Abstract

Foot-and-mouth disease (FMD) is an economically important contagious viral disease that affects livestock species such as cattle, sheep, and goatspigs (Terrestrial code of the World Organization for Animal Health, OIE). Egypt, Canada, and many countries in Europe and Asia, although currently free of FMD virus, are at risk for new outbreaks, due to their non-vaccination policy, allowing the livestock population in these countries to become highly susceptible. The risk of a new outbreak is not hypothetical as illustrated by the large epidemic of FMD in 2008 in Europe, which started in the United Kingdom, a country often considered to have a lower risk for virus introduction than mainland Europe. The virus spread to France, the Netherlands, and Ireland, and resulted in the slaughter of millions of animals. The UK applied the regular EU measures, like culling of infected herds, pre-emptive culling of contiguous farms, and control of movement. These measures were also implemented in the Netherlands, but in addition, emergency vaccination of the whole susceptible livestock population in the affected area was applied, followed by culling of all vaccinated animals. So from that research we work to face the outbreaks by emergency vaccine production which will be include viz., � Thermo stability of the vaccine, b) � Give highly Duration of immunity, effective and safe adjuvants, c) � Broad antigenic coverage for the emerging viral strains, c) � Ease of administration for mass coverage, d) � Cost effective scale up without dependence on expensive infrastructure, e) � DIVA enabled technology, f) � Common vaccine platform to accommodate newly emerging field strains to address region specific epidemiology and g) Working on � Improvements to conventional vaccines � Several approaches for new generation vaccines: expression of VP1, P1-2A, P1-2A-3C attempted in several platforms including bacteria, yeast d � Development of DNA vaccines & nanoparticle delivery. The By 1. Evaluation of FMD vaccine formulated with novel adjuvants 2. Development of adenovirus vector based FMD vaccine (USDA-IVRI-PDFMD) 3. Using baculovirus expressed capsid proteins as candidate vaccine 4. Developing stable vaccine virus for use with conventional vaccine (infectious cDNA approach) Evaluation of high pay load vaccine using two adjuvants (A, B) � Pay load used Otype A and Sat2 � 2ml dose by intramuscular and subcutaneous � 8 animals per group (9 groups including control) � Humoral response by SNT at regular intervals till 9 months � Booster vaccination in three groups at 6 months � Results based on SN Ab response are encouraging � Booster dose induced a robust Ab responses in all groups. � Study is being continued to determine the duration of immunity by SNT and challenge at 1 year. � Additional studies for optimizing payloads planned FMDoot and mouth disease is capable of infecting cattle, sheep,goats and pigs. The disease has high morbidity, but low mortality and is highly contagious. But, vaccination of lower-producing animals may also be justified, especially where these animals produce milk or traction power, or where this would serve to protect high-producing livestock from disease challenge. Moreover, FMD is probably the most important livestock disease in terms of economic impact. The disease causes the greatest production losses in ruminants, and in particular in intensive dairy systems. FMD status is an important determinant of international trade in livestock products, and the existence of FMD is an effective barrier from the markets with the highest prices for these products. Therefore, many resources have been and still are dedicated to surveillance, control and eradication of this disease. These efforts have been successful in that many areas of the world are now either free from FMD or have the disease under control. The incentives of these control activities are dependent on the export potential of countries and the types of livestock systems that are found within the countries. Keywords: Ffoot and Mmouth Ddisease, Livestockimpact of foot-and-mouth disease, Dairy, Production, , effect of FMD

Biography

Dr. Amany Mohamed Abbas work as a researcher in Veterinary serum and vaccine research institute as a quality Assurance Manager, technical manager deputy and head of virology unit in Quality Control Laboratory. Researcher of virology Department till now. Professional experience includes Preparation of tissue culture BHK21 ,VERO, MDBK cell lines and primary cell culture MDCK ,CEF,OS, Preparation and evaluation of attenuated and inactivated bovine viral disease Vaccines, Evaluation of different viral disease vaccines according to international protocols, Evaluation of post vaccinal immune response by using different serologicaltests, preparation of some nano- adjuvants, molecular RT-PCR, Formulating program of training courses in virology and immunology for veterinarians and technicians, training on Principals of Bioinformatics and Biological Data Analysis, ISO 17025: 2005- 2017, ISO 22000 lead auditor, Trained on molecular diagnostic at the Vector and Vector Borne Disease Research Institute, Tanga-Tanzania.

Speaker
Amany Mohammed Abbas Abd Elkader / Researcher at Quality Control Laboratory, Egypt

Plenary Talks

Abstract

The use of nanomaterials in medicine has gained tremendous popularity in recent years, and many potential applications have been suggested. One promising option is their use in the field of medical imaging. Nanomaterials have shown to affect the signals used for image formation even at very low concentrations. Hence, their ability to serve as contrast enhancing agents has been explored. In this talk the mechanisms responsible for contrast generation in: Ultrasound, MRI, and CT will be initially reviewed. Then, the motivation for multimodal imaging will be explained. Finally, detailed description of several projects of our group implementing metallic nanoparticles based materials including: Iron, Copper and Gold for dual modal Ultrasound-MRI and MRI-CT imaging will be described.

Biography

Prof. Haim Azhari, was born in Jerusalem, Israel in 1955. He received his B.Sc in Mechanical Eng. from the Technion in 1977, his M.Sc. (Cum Laude) in Biomedical Eng. from Tel-Aviv University 1984, and his D.Sc. in Biomedical Eng. from The Technion in 1987. From 1987 to 1990 he was on the staff of the Technion Department of Biomedical Engineering in a postdoctoral position. Prof. Azhari then received a double appointment as an International Research Fellow in both the Department of Radiology and the Division of Cardiology at the Johns Hopkins School of Medicine in Baltimore Maryland USA, and was in the USA from 1990 to 1992. On his return in 1992 he was appointed Senior Lecturer in the Department of Biomedical Engineering Technion-IIT. From 1999-2000 he was on a Sabbatical leave at Harvard Medical School at Beth-Israel radiology department at Boston Massachusetts. Between 2011-2015, he was the president of the Israeli Society for Medical and Biological Engineering (ISMBE). His Field of research is Medical imaging, which includes: Multimodal imaging. Application of Magnetic Resonance Imaging (MRI) and Ultrasound in medical imaging. Algorithms and methods for image reconstruction. Image processing, and information fusion and extraction from medical images.

Speaker
Haim Azhari / Technion - Israel Institute of Technology, Israel

Abstract

Biography

Director of the postdoc school in a CEEX project entitled „Micro and nanostructured elaborated via chemical and electrochemical bioactivation with application in regenerative medicine.Expert evaluator and monitor of national (RELANSIN, MENER, BIOTECH, MATNANTEC) and international projects (LEONARDO Da VINCI, CORINT, Czech Science Foundation, Invited lecture or/and chair person in Conferences and Universities (SUA, Poland, Mexic, Greece, 2010 Hong Kong (2010), Egipt, France, Kuala Lumpur,Great Britain, Denmark (2014), Italy, China (2015), China 2016The Netherlands (2016),Prague 2017, JEJU ( KOREEA ) 2018. Expert in POSDRU Projects. Expert in UNIDO, FP6 and Leonardo da Vinci projects. Member of Romanian Chemistry Society, member of Romanian Biomaterials Society, member ISI ( International Electrochemistry Society), Member of Committee of APCBEES( Asia Pacific Chemistry Biological & Environmental Society), member of International Bionic Society. member of editorial board for Nanobiomedicine (Japan) and of J.of Roumanian Biophysics editor for Coatings ( Special Issue) Micro and Nanocoatings for technological and biomedical applications .Reviewer for J.Nanoparticles research, Biomaterials, Corr. Sci., Electrochimica Acta, J. Non Crystalline solids, Appl. Surf. Sci., Surf. and Coating Tech, Rev. Chim., J.Synthetic metals, Arab.J.of Chemistry, Non crystalyne solids, Mater Chem. and Phys, J. Biosci. Bioeng., J. Mater. Sci. Mater. Medicine, J. Colloid Interface B, Sci Reports J. Nanomaterials, J of Ceramics International. Chairperson and/or member in scientific committee International Conferences Romania, (RICCCE 2005,2007,2009,2011), Greece (Duracosys 2010), Egipt(2011), Hong Kong(2010), Thailanda (International Conference on Biotechnology and Environment Management - ICBEM 2012), Denmark (International Conference on Biomedical Engineering and Technology ICBET May 2013 Copenhagen) France (Medical and Bioscience Conference Paris October 2013),Great Britain (2014 4th International Conference on Environmental, Biomedical and Biotechnology, ICEBB 2014 July Nottingham) Italy (May 2015 Florence),The Netherlands (Amsterdam March 2016), Prague 2017 China (5th International Conference on Bionic Eng. 2016), etc. Awards -Silver medal at Geneva International Saloon of Inventions ( 2002) .WOMPI premium at Geneva International Saloon of Inventions ( 2011 ).Premium of Romanian Society of Biomaterials Daniel Bunea 2011.Over 50 papers have CNCS prize, the majority of them being from the red zone and having influence score higher than 1 (Ioana Demetrescu as principal author). Honorary Professor „Dunarea de Jos” University .Corespondent member of Academy of Romanian Scientists. Articles 192 ISI publications starting 1977 and 1600 citations. Research projects:Participation in more than 100 projects at national and international level including, UNIDO, Leonardo da Vinci, FP6, CNCSIS, VIASAN, CEEX, PCE, PCCE, etc ; in the period 2006-2019 Director of 15projects, (2 international cooperation with France and Argentina in the field of biomaterials and 13 national). International cooperation France, Spain,Greece, Germany, Japan, Portugal, Argentina, Findland. Publications : Books Participation in elaboration of 18 books including chapters at international level. Patents 9 patents in materials Science.

Speaker
Demetrescu Ioana / Politehnica University of Bucharest, Romania

Abstract

Chirality is one of life’s most distinctive biochemical signatures and has great influence on many biological events, e.g. maintaining normal functions for living cells. It reveals that cells can sense surface chiral molecules to show differential behaviors on enantiomorphous surfaces. So far, the researches are mainly confined to the role of molecular chirality on two dimensional (2D) surface and a lot of questions remaining to be answered. Among them, how nanofibrous chirality influences cell behaviors in three dimensional (3D) extracellular matrix (ECM) is especially important, since it is only the 3D ECM nanofibrous structure can really mimick the necessary biophysical environment for tissue engineering and helical nanofibrous structure is closely related with the relevant biological events.To explore this, supramolecular gelators are of particular interest candidate because their assembly arises from non–covalent interactions. With the rational design of chemical composition and molecular structures, surpramolecular gelators can be efficiently self-assemble into two or three dimensional chiral microstructures, showing a big potential as biomimetic scaffold for multi-dimensional cell culture. With variation of physical or chemical properties, the chiral structures with the varied surface composition, mechanical strength, and surface wettability can be constructed and chirality regulated cell adhesion can be obtained in 3D. It is found that left-handed hydrogels can enhance cells adhesion and proliferation, but not for right-handed hydrogels. A smart control of cell adhesion is also realized by applying external fields, such as light, pH and so on. The study paves a way to explore the influence of chiraity of nanostructures on cell behaviors cell culture in 3D chiral environments and this chiral materials have potential application in the field of tissue engineering. For example, recently, stem cells are successfully cultured in 3D and it is found that stem cells can successfully differentiate into Osteoblast, however, stem cells are inhibited to differentiate.

Biography

He is currently the deputy director of the National Biomedical Materials Engineering Technology Research Center. His main academic part-time jobs include the director of the China Biomaterials Society, the deputy director of the Bone Repair Materials and Devices Branch, and the deputy director of the 3D Printing Branch of Biomedical Materials . 1989 to 1997 , has participated in the national eight-five, nine-five research projects, engaged in high-performance high-temperature polymer materials and composite materials research work, and undertake the work of military supporting materials projects, and achieved a number of results He has won the " National Eighth Five-Year Key Achievements " Award and the " Ministry of Science and Technology Progress Third Prize " .   Since 2001 , he has participated in research on polylactic acid synthesis and chemical recycling in major projects of the Ministry of Education, Culture, Sports, Science and Technology. He has developed research on synthesis, modification and decomposition properties and chemical recycling of biodegradable polymer polylactic acid. An environmentally friendly catalytic system. In 2004 , he entered the Biomaterials Center of the National Materials Research Institute of Japan, using biodegradable and absorbable polymer materials for drug release systems and tissue engineering research. In 2006 , he returned to China to join the Biomaterials Engineering Research Center of Sichuan University and the National Biomedical Materials Engineering Technology Research Center. As project leader, has hosted the National High Technology Research and Development Program ( 863 Program) key project 1 , the National Science and Technology Support Program 2 , the National Natural Science Foundation project project 1 , the National Natural Science Foundation of China 3 Xiang And provincial and ministerial level scientific research projects. Published more than 80 SCI papers, with more than 1,000 citations . Apply for more than 30 invention patents in China and authorize more than 10 patents . Apply for 3 Japanese patents , two of which filed PCT international patent applications and applied for patent licenses in several countries. Development of collagen-based cartilage repair products (in the clinical stage) and bioactive toothpaste (pre-production phase) and other products.

Speaker
Fan Yujiang / Sichuan University, China

Abstract

Biography

Professor Haitao Ye joined the Department of Engineering at the University of Leicester as the Chair in Materials Engineering in April 2018. Prior to that, he was a Reader and Nanoscience Research Group Convener at Aston University, Birmingham. He was employed as Research Fellow /Senior Research Fellow at London Centre for Nanotechnology, University College London, following the completion of an Industrial Research Fellowship at the Basic Research Labs of Nippon Telegraph & Telephone Corporation (Japan).

Speaker
Haitao Ye / University of Leicester, UK

Abstract

Biography

Masaru Tanaka received his PhD degree from Hokkaido University in 2003. In the period of 1996-2000 he worked for TERUMO Co. and designed novel biocompatible polymers and commercialized as an artificial lung. In 2000 he moved to Hokkaido University and in 2007 he moved to Tohoku University. Stents covered with the self-organized porous 3D films are commercially available in the world clinical market. In 2009 he was awarded a full professorship at Yamagata University. From 2015, he is at Kyushu University as a Professor. He has received the SPSJ Asahi Kasei Award for his intermediate water concept based on the role of interfacial water at materials’ interphases. Intermediate water content is a good predictor of biological responses to materials and is using for high-through put materials discovery.

Speaker
Masaru TANAKA / Kyushu University, Japan

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