Call Us: +91-779-979-0002/+91-779-979-0003

Plenary Talks

Abstract

Dr. Miguel Nogueras(CLS, ASCP), RABQSA Director of J&J – Sterility Assurance Johnson & Johnson Franchise Leader – Consumer Medical Device (CMD) To help ensure patient safety and product quality, the focus for Sterility Assurance professionals for over 40 years has been to deliver sterile products for use by nurses, doctors, and other healthcare providers to help reduce the potential for patient infections. As we look in to the future, there are many new frontiers in healthcare, which requires the sterility assurance community to adapt our methods and engagement to an ever-increasing rate of innovation. While the development of new drugs and devices used to take a decade, it can now only happen in just a few years. To ensure more efficient delivery of these new innovations to patients, sterility assurance needs to keep pace with these product innovations. This can only be achieved by participating at all stages of the product life cycle rather than just focusing on a sterilization technology or modality. Sterilization cannot be an afterthought in product innovation, and sterility assurance professionals need to have a place at the table from design throughout the total life cycle of a product. No matter how innovative or groundbreaking a product might be, it’s rendered useless if not properly designed to withstand the sterilization, reprocessing and disinfection challenges we face today. Too often, sterility assurance comes at the end of the R&D process, mainly during the transfer process into production. If we are not innovative, we can either stop a product from coming to market or we could seriously delay the ability to bring new products to the market, thereby delaying advances that bring value to patients and/or consumers. To help promote collaborative innovation, the sterility assurance community needs to leverage its scientific foundation to constantly question the status quo, thereby opening doors to innovation. In order to achieve this, we need to start incorporating our professional in all steps of the process. During the Sterility Assurance E2E presentation we will explore the advantages and overcome challengesby artifact of incorporating the model into the product design/development, life cycle and distribution.Incorporating sterility assurance professionals in such a way will demonstrate: faster incorporation of new technology, and faster regulatory approvals servicing our patient needs sooner while ensuring patient safety.

Biography

Dr. Nogueras joined Abbot Medical Optics (AMO) now J&Jin 2009 as Divisional Sr. Manager of Quality & Compliance in the areas of QC, Microbiology, Environmental Controls and Sterilization. He was also one of the founders and chair of AMO Global Microbiology Council and Co-Founder of the Abbott Sterilization Task Force.He has held multiple positions within J&J including Site Quality Director at several of our manufacturing sites including Añasco, Puerto Rico, Kulim, Malaysia, and Hangzhou, China. Miguel has over 20 years of experience in the industry, including clinical research, pharmaceutical, viral vaccines, biological products, autologous molecules and medical devices. Miguel holds a Doctorate in Viral Pathology and Infectious Diseases from the University of Paris- L’Institut Louis Pasteur, and a B.S. in Industrial and Clinical Microbiology from the University of Puerto Rico-Mayagüez Campus. Heis an adjunct faculty member at multiple academic institutions throughout the world. Dr. Nogueras is a recognized industry leader in sterility assurance and sterilization practices, and he has over 20 years’ experience in sterility assurance.

Speaker
Dr. Miguel Ángel Nogueras / Director at Sterility Assurance for the Consumer Medical Device Group (CMD) at Johnson & Johnson

Abstract

There is increasing evidences that favor the prenatal beginning of schizophrenia. These evidences point toward intra-uterine environmental factors that act specifically during the second pregnancy trimester producing a direct damage of the brain of the fetus [1]. The current available technology doesn't allow observing what is happening at cellular level since the human brain is not exposed to a direct analysis in that stage of the life in subjects at high risk of developing schizophrenia. Methods. In 1977 we began a direct electron microscopic research of the brain of fetuses at high risk from schizophrenic mothers in order to finding differences at cellular level in relation to controls. Results. In these studies we have observed within the nuclei of neurons the presence of complete and incomplete viral particles that reacted in positive form with antibodies to herpes simplex hominis type I [HSV1] virus, and mitochondria alterations [2]. Conclusion. The importance of these findings can have practical applications in the prevention of the illness keeping in mind its direct relation to the aetiology and physiopathology of schizophrenia. A study of the gametes or the amniotic fluid cells in women at risk of having a schizophrenic offspring is considered. Of being observed the same alterations that those observed previously in the cells of the brain of the studied foetuses, it would intend to these women in risk of having a schizophrenia descendant, previous information of the results, the voluntary medical interruption of the pregnancy or an early anti HSV1 viral treatment as preventive measure of the later development of the illness.

Biography

Segundo Mesa Castillo. As Specialist in Neurology, he worked for 10 years in the Institute of Neurology of Havana, Cuba. He has worked in Electron Microscopic Studies on Schizophrenia for 32 years. He was awarded with the International Price of the Stanley Foundation Award Program and for the Professional Committee to work as a fellowship position in the Laboratory of the Central Nervous System Studies, National Institute of Neurological Diseases and Stroke under Dr. Joseph Gibbs for a period of 6 months, National Institute of Health, Bethesda, Maryland, Washington D.C. USA, June 5, 1990. At present he is member of the Scientific Board of the Psychiatric Hospital of Havana and give lectures to residents in psychiatry

Speaker
Dr. Segundo Mesa Castillo / Psychiatric Hospital of Havana.

Abstract

Biography

Minić Ivan is very enthisuistatic Researcher in field of Medical science .He Actively participates in all world wide conferences and actively participating as Editorial Board Member in more than 15 Journals .He gave wide contribution from his side.

Speaker
 Dr. Minic Ivan / Medical Faculty, University of Nis, Serbia

Sessions:

Microbiology and Parasitology

Abstract

Innovation by the Incorporation of the End to End (E2E) Sterility Assurance Concept Dr. Miguel Nogueras(CLS, ASCP), RABQSA Director of J&J – Sterility Assurance Johnson & Johnson Franchise Leader – Consumer Medical Device (CMD) To help ensure patient safety and product quality, the focus for Sterility Assurance professionals for over 40 years has been to deliver sterile products for use by nurses, doctors, and other healthcare providers to help reduce the potential for patient infections. As we look in to the future, there are many new frontiers in healthcare, which requires the sterility assurance community to adapt our methods and engagement to an ever-increasing rate of innovation. While the development of new drugs and devices used to take a decade, it can now only happen in just a few years. To ensure more efficient delivery of these new innovations to patients, sterility assurance needs to keep pace with these product innovations. This can only be achieved by participating at all stages of the product life cycle rather than just focusing on a sterilization technology or modality. Sterilization cannot be an afterthought in product innovation, and sterility assurance professionals need to have a place at the table from design throughout the total life cycle of a product. No matter how innovative or groundbreaking a product might be, it’s rendered useless if not properly designed to withstand the sterilization, reprocessing and disinfection challenges we face today. Too often, sterility assurance comes at the end of the R&D process, mainly during the transfer process into production. If we are not innovative, we can either stop a product from coming to market or we could seriously delay the ability to bring new products to the market, thereby delaying advances that bring value to patients and/or consumers. To help promote collaborative innovation, the sterility assurance community needs to leverage its scientific foundation to constantly question the status quo, thereby opening doors to innovation. In order to achieve this, we need to start incorporating our professional in all steps of the process. During the Sterility Assurance E2E presentation we will explore the advantages and overcome challengesby artifact of incorporating the model into the product design/development, life cycle and distribution.Incorporating sterility assurance professionals in such a way will demonstrate: faster incorporation of new technology, and faster regulatory approvals servicing our patient needs sooner while ensuring patient safety.

Biography

Dr.Miguel Ángel Noguerasis the Director, Sterility Assurance for the Consumer Medical Device Group (CMD) at Johnson & Johnson. In this role, Miguel is responsible for end to end implementation of sterility assurance policies and strategies and assures that CMD programs are aligned with the overall J&J Sterility Assurance programs. He will serve as the global technical leader in the areas of aseptic processing, sterilization and microbiology across all CMD locations worldwide. Dr. Nogueras joined Abbot Medical Optics (AMO) now J&Jin 2009 as Divisional Sr. Manager of Quality & Compliance in the areas of QC, Microbiology, Environmental Controls and Sterilization. He was also one of the founders and chair of AMO Global Microbiology Council and Co-Founder of the Abbott Sterilization Task Force.He has held multiple positions within J&J including Site Quality Director at several of our manufacturing sites including Añasco, Puerto Rico, Kulim, Malaysia, and Hangzhou, China. Miguel has over 20 years of experience in the industry, including clinical research, pharmaceutical, viral vaccines, biological products, autologous molecules and medical devices. Miguel holds a Doctorate in Viral Pathology and Infectious Diseases from the University of Paris- L’Institut Louis Pasteur, and a B.S. in Industrial and Clinical Microbiology from the University of Puerto Rico-Mayagüez Campus. Heis an adjunct faculty member at multiple academic institutions throughout the world. Dr. Nogueras is a recognized industry leader in sterility assurance and sterilization practices, and he has over 20 years’ experience in sterility assurance.

Speaker
Dr. Miguel Nogueras(CLS, ASCP), RABQSA / Director of J&J – Sterility Assurance Johnson & Johnson Franchise Leader – Consumer Medical Device (CMD)

Abstract

There is increasing evidences that favor the prenatal beginning of schizophrenia. These evidences point toward intra-uterine environmental factors that act specifically during the second pregnancy trimester producing a direct damage of the brain of the fetus [1]. The current available technology doesn't allow observing what is happening at cellular level since the human brain is not exposed to a direct analysis in that stage of the life in subjects at high risk of developing schizophrenia. Methods. In 1977 we began a direct electron microscopic research of the brain of fetuses at high risk from schizophrenic mothers in order to finding differences at cellular level in relation to controls. Results. In these studies we have observed within the nuclei of neurons the presence of complete and incomplete viral particles that reacted in positive form with antibodies to herpes simplex hominis type I [HSV1] virus, and mitochondria alterations [2]. Conclusion. The importance of these findings can have practical applications in the prevention of the illness keeping in mind its direct relation to the aetiology and physiopathology of schizophrenia. A study of the gametes or the amniotic fluid cells in women at risk of having a schizophrenic offspring is considered. Of being observed the same alterations that those observed previously in the cells of the brain of the studied foetuses, it would intend to these women in risk of having a schizophrenia descendant, previous information of the results, the voluntary medical interruption of the pregnancy or an early anti HSV1 viral treatment as preventive measure of the later development of the illness.

Biography

Segundo Mesa Castillo. As Specialist in Neurology, he worked for 10 years in the Institute of Neurology of Havana, Cuba. He has worked in Electron Microscopic Studies on Schizophrenia for 32 years. He was awarded with the International Price of the Stanley Foundation Award Program and for the Professional Committee to work as a fellowship position in the Laboratory of the Central Nervous System Studies, National Institute of Neurological Diseases and Stroke under Dr. Joseph Gibbs for a period of 6 months, National Institute of Health, Bethesda, Maryland, Washington D.C. USA, June 5, 1990. At present he is member of the Scientific Board of the Psychiatric Hospital of Havana and give lectures to residents in psychiatry

Speaker
Dr. Segundo Mesa Castillo / Psychiatric Hospital of Havana.

Abstract

Current research strongly indicates that male circumcision has health benefits. We Jews circumcise only our sons and we forcefully disapprove female genital mutilation/cutting/circumcision. In a personal communication to me, Harald zur Hausen (Medicine Nobel Prize 2008) does state that the effect of male circumcision in protecting against sexually transmitted diseases is at best moderate. In my texts discussing health benefits of male circumcision, human papillomavirus (HPV) is very much in focus. Male circumcision is linked to decreased risk in patients with penile HPV infection and penile cancer, and is related to reduced risk of cervical cancer in their female sexual partners.

Biography

I was born in Turku, Finland in 1933. My own language is Swedish. I have completed my MD from University of Lund, Sweden in 1962 and my PhD from University of Uppsala, Sweden in 1981. My main scientific subject is human papillomavirus (HPV) infection – male circumcision in focus.

Speaker
Elias Rubinstein MD PhD /

Abstract

Abstract Purpose: Malaria remains the most devastating infectious parasitic disease responsible for the death and economic losses among half the world’s population. The persistent development of resistance to most modern antimalarial drugs by Plasmodium species has necessitated the search for active ingredients in plants with antimalarial activity. The stem bark of Pseudocedrela kotschyi is used locally for the treatment of fever and the crude extracts have been reported to demonstrate antimalarial activity, hence the need for this research to authenticate the aforementioned claim. The aim of this study is to isolate and determine the antimalarial activity of fractions of the crude stem bark extracts of P. kotschyi using the rodent model. Materials and Methods: 200grammes of the stem powder was extracted in a soxhlet extractor using ethanol. 12 grammes from the ethanol extracts was pre adsorbed on silica gel using Thin layer chromatography (TLC) to determine the appropriate solvent system to be used for flash chromatography method which properly separated the component into 19 eluents. Fractions 1-4, 6–7, 10–11, 12-19 were combined, purified by repeated preparative TLC that recrystallized to give 7 fractions. Each of the fractions was subjected to GC-MS analysis. 40 mice inoculated with Plasmodium berghei berghei were divided into 10 groups of 4 animals and treated with 100 mg/kg of the fractions to evaluate the antimalarial activity of fractions using suppressive and curative tests. Results: Fraction 5 recorded the highest chromatogram profile with an intensity of 13,260,430 while fraction 9 had the lowest chromatogram profile of 1,334,989. GC-MS analysis showed the presence of forty-four (44) chemical compounds in all the fractions of the plant. The fractions at 100 mg/kg suppressed the parasites by 100 %, 93.73%, 84.08 %, 78.15 %, 77. 93 %, 68.24 % and 57.06 % in fractions 5, 6-7, 10-11, 8, 1-4, 12-19 and 9 respectively while the crude extract produced 73.95 % suppression. The curative test recorded significant decrease in fractions 8, 9, 10-11, 12-19 and the crude extract. Conclusion: P. kotschyi contain active ingredients that have suppressive antimalarial which could be further harnessed by the pharmaceuticals industry to curb the menace of malaria in the world. Keyword: Antimalaria , Pseudocedrela kotschyi, Plasmodium berghei berghei

Biography

Prof David P. Yakubu has completed his PhD from University of Nottingham, U.K. in 2005. He is the Director of Student Industrial Work Experience Scheme (SIWES). He has published more than 25 papers in reputed journals and has been serving as peer reviewer of repute. Anthony Dawet has completed his PhD in 2014 from University of Jos. He is a Senior Lecturer in the Department of Zoology. He has published more than 15 papers in reputable journals and has been peer reviewer of repute. R. Omagha has completed her M Sc. from University of Jos in 2015 and is currently studying her PhD in University of Lagos, Nigeria.

Speaker
D.P.Yakubu / Department of Zoology, Faculty of Natural Sciences, University of Jos

Abstract

We conducted comparative studies and analysis by means of transmission, scanning, comparative electron microscopy research and analysis of some types of bacteria (E.coli, Salmonella,Vibrio,Lactobacillus, et al), yeast (Candida) and Entamoeba. Based on obtained results, we suggest a study of the major changes in prokaryotic(gram negative,gram positive) and eukaryotic cells(yeasts,protists) ultrastructural pathologies patterns during culturally grown and different of the action of certain physical, chemical and biologically active natural factors on the ultrastructural characteristics of bacteria and protists (antibiotics, disinfectant, biologically active substrates(BAS), ultraviolet, X-radiation,γ-ionizing radiation. We have conditionally divided the microbes, yeasts and protozoa cell pathology changes into adaptive, balanced and irreversible-destructive ones. The convenient and destructive types of structural changes can be observed within the same dose of the same preparation or factor. The adaptive changes in bacteria are manifested by the formation of spheroplasts, protoplasts, L-form, non-cultivation dormants forms,lysogen and nanobacteria. Convenient changes in protozoa are expressed in plasmic layer, and cytoplasmic ribonucleoproteidal plasma, glycogenic, volutin structural changes, virus-like noncultivation symbiont, as well as with the increase in the number of phagocytosis and pinocytosis vacuoles and by the disintegration of chromatin. The suggested cell disease classification of bacteria, yeasts, and protozoa can be useful for bactericidal, sporicidal, protistocid preparations researches, as well as for the research of mechanism of interactions with microorganisms.

Biography

Speaker
Dr. Karlen / Center of Technological Organic and Pharmaceutical Chemistry of NAS RA, 26 Azatutyan Str. 0014,Yerevan, 0014, Armenia

Abstract

Some plant diseases that can completely destroy Potato plot, late blight is one of these devastating fungal diseases that attacks the potato field and completely destroy the potato field. If any proper preventive measure cannot be taken from beginning of initiate the late blight, farmers as well as potato seed production companies can be faced into great hamper. Although this devastating diseases can be controlled by using traditional fungicides like Carbondazim or Metalaxyl. Plant growth and tuber formation are gradually inhibited. As a results the farmers and seed potato production companies cannot get sufficient production/ expected yield. The objective of this present research is to standardize the protocol by which late blight diseases can be controlled easily without inhibition of the plants growth and its yield. Efforts made in present investigation to evaluate the concentration of few commonly used fungicides for their comparative efficacy against Phytophthora infestans (Mont.) de Bary- the incited of late blight, for its The highest (99.70) percentage of disease control and the highest yield (26.68 MT/ha) were recorded on fungicides containing 3.5 mg/L Sunoxanil 72 WP (Cymoxanil 8%+Mancozeb 64%) with 3.0 ml/L Contaf 5 EC (Hexaconazol 5%) during 2015-2016. The lowest (75.68) percentage of disease control and the lowest yield (15.67 ton/ha) were recorded on fungicides containing 2.0 mg/L Ridomil MZ 72 (Metalaxyl 8%+Mancozeb 64%) with 1.0 ml/L Autostin 50 WDG (Carbondaxim 50%) during 2014-2015. It was concluded that Sunoxanil 72 WP (Cymoxanil 8%+ Mancozeb 64%) acted as the best fungicide when applied as prophylactic measures. Sunoxanil 72 WP (Cymoxanil 8%+ Mancozeb 64%) was combined with Contaf 5EC (Hexaconazol 5%) or Actiphose (Phosphorous acid) showed the best result when applied as curative measures. Plant growth as well as its yield gradually decreased due to the application of Carbondaxim and Matalaxyl. It is new finding, although Metalaxyl and Carbondaxim play on vital role to protect the late blight after appearance the disease, the plant growth, tuber formation along with the yield was gradually inhibited. Therefore, it is recommended that Metalaxyl and Carbondaxim application should be avoided to protect the late blight after or before appearance the disease.

Biography

Author has done Ph.D. in Plant Biotechnology especially tissue culture and has published quite number of scientific publications in highly reputed journals in home and abroad. His Ph.D. dissertation was published as a text book from LAP LAMBERT Academic Publishing, Germany and another book entitle “Comparative Efficacy of Different Fungicides against Late Blight Diseases of Potato incited by Phytophthora infestans (Mont.) de Bary and standardized the doses for its Management” was also published from LAP LAMBERT Academic Publishing, Germany. He has an experience in tissue culture laboratory as well as has an experience to ensure quality of potato seed production farm especially in mini-tuber and breeder seed of potato production within net house and foundation and certified seed of potato production in field.`

Speaker
Siddique N.A / Siddique N.A, Ph.D, Metal Agro Ltd, Dhaka, Bangladesh,; Study on Rajshahi University, Bangladesh.

Abstract

Cutaneous Leishmaniasis (CL), caused by several Leishmania species, is a complex disease with a wide spectrum of clinical features. This neglected vector-borne disease, is a major public health problem worldwide and one of the most common endemic diseases in Iran. According to the World Health Organization (WHO) reports,about 900,000 to 1.3 million new cases of leishmaniasis are reported per year; of these, approximately 0.7–1.2 million are cutaneous leishmaniasis. It is estimated that 20000 new cases are reported annually in Iran, nevertheless, due to under-reporting,theactual number is almost higher. North Khorasan province in north eastern Iran is one of the important focus of CL, and this study was performed to determine the main parasites, reservoir hosts and vectors of the disease in this district. Leishmania positive isolates from human cases in North Khorasan province (North East of Iran),were genotyped using PCR-RFLP analysis. Wild mammals were caught from gerbil burrows and the genus and species of the rodents were determined by external morphological characteristics. Leishmania parasites were detected to assess the infection of reservoir hosts through microscopically methods, PCR-RFLP and phylogenetic analysis. Sand flies were collected by sticky papers and identified to species level using light microscope and keys. PCR of kDNA, ITS1-rDNA, followed by RFLP were used for identification of DNA of Leishmania parasites within infected sand flies. The results of this study indicated that the parasite causing cutaneous leishmaniasis in this province is L. major. The results also revealed that Rhombomysopimus is considered as the main reservoir host of CL. Microscopic investigation on blood smear of the animals for amastigote parasites and PCR-RFLP revealed the presence of L. major in rodents. Two species of Phlebotomuspapatasi and Phlebotomussalehiwere found to be naturally infected with L. major. Moreover, mixed infection of L. turanica and L. major was observed in one specimen ofP. papatasi. Characteristics of the collected Leishmania isolates from North Khorasanrevealed that L. major is a predominant parasite of cutaneous leishmaniasis. Our study revealed that R. opimusis the most important reservoirhost for maintenance of the parasite source in the area. According to the results, two vectors of cutaneous leishmaniasis were found to be active in studied area. P.papatasi and P. salehiare, like in several places, the probable vectors of cutaneous leishmaniases in this focus of cutaneous leishmaniasis. We also found that the disease is endemic in Northern Khorasan Province.

Biography

Speaker
SadafSabzevari /

Abstract

Rheumatic fever and Endomyocardial fibrosis (EMF) are two distinct expressions of the same pathological process as a result of immunological reactions due to cardiotrophic agents. Group A streptococcal infection causes acute rheumatic fever, leading to pancarditis and valvular dysfunction. Similar to rheumatic fever, EMF predominates in children and young adults, causing extensive fibrosis of endocardium with plaque-like lesions due to various infections and it has been challenging to identify acute phase, characterized by generalized allergy/ immunological features including fever, abdominal distension, facial and periorbital swelling, urticaria, myopericarditis and neurological features. These two neglected cardiovascular diseases are confined to limited geographical areas, carries grim prognosis and emphasizing alternative routes for understanding and treatment of the disease.

Biography

will update soon

Speaker
Dr. Ramachandran Muthiah, M.D.,D.M., FNB, ACCF / Consultant Physician & Cardiologist

Abstract

The root-knot nematode (RKN) Meloidogyne incognita is considered one of the most economically harmful pathogens to worldwide agriculture and devastating to coffee plantations. Genetic improvement strategies for introgression of R genes result in a breakdown of resistance, whereas nematicidal chemicals are considered to be inefficient or toxic. In previous works, we observed that a gene homologous to miraculin was overexpressed at 5 and 6 days after infection in coffee roots resistant to M. incognita. Samples of these roots were sequenced by RNAseqllluminaHiSeq 4000, generating more than 800 million length readings of 2x100 nt. Differential in silico expression analysis and GO enrichment results indicate that some gene families are strongly deregulated in the resistance response. Fourteen superexpressed genes of Kunitz type family protease inhibitors (PIs), similar to miraculin, have been identified. The evidence to date is that miraculin must be important in responding to coffee stresses, because the CoMirmiraculin gene is overexpressed by the attack of the coffee beetle (Leucopteracoffeella). The present study shows the real-time expression analysis of the miraculin-like genes in coffee and the correlation of their expression patterns to other biotic or abiotic stress responses. These genes may be important actors in the coffee immune response and may be potentially used in biotechnology approaches for the control of nematodes and pests.

Biography

PhD. at the Science Faculty of Université de Montpellier II - France, and postdoctoral studies in nematode genomics at INRA (Sophia Antipolis - France). Currently principal investigator at the Brazilian Agricultural Research Corporation. Acts in phytopathology researching plant-pest interactions, especially with nematodes and insects. Has experience in the field of Biochemistry, with emphasis in Molecular Biology, studying mainly the following subjects: genomic, transcritômica, functional validation of genes, silencing by interfering RNA, genetic transformation of plants, genetic editing of plants.

Speaker
Érika Albuquerque / Embrapa Recursos Genéticos e Biotecnologia, Brasília - Brazil

Abstract

Title: Intestinal parasitoses in urban and rural children of a developing country. Objective: To compare intestinal parasitoses between urban and rural children in a developing country and its related factors. Methods: Children of 1-6 years old in the urban and rural areas who had not taken antihelmenthic drugs in the last 6 months were randomly selected. Various factors were taken as variables in a preformed questionnaire. Results: Among the total 52 children, 24 were in urban and 28 were in rural group. Male/female ratio of the rural group was 1:1 and that of the urban group was 1.4:1. Average family income was 12000 taka and 4000 taka per month in urban and rural areas, respectively. None of the rural group used sanitary latrine, whereas every one in urban group used sanitary latrine. Majority (71%) of the mothers in rural group were below the primary level of education. Majority (75%) of fathers were educated above higher secondary level in urban group. In the rural group 18 (64%) fathers had none received any education. Enterobius vermicularis was observed only in 1(4%) stool samples of urban children but in rural group it was found in 7(25%) of the stool samples. Enterovirus vermicularis was the major parasite isolated from both groups. Exclusive breast feeding practice of less than 6 months was observed in 20 (83%) children of urban group but in rural group that of less than one year was found in 22 (78%) children. Conclusion: Intestinal parasitic infestation is mostly found in rural children. Urban children almost have no intestinal parasitoses. It has the relation with the poor sanitation, low standard of living, less parental income and education, and early weaning. Unnecessary universal deworming of the urban and rural children in developing countries should be discouraged in order to use the money economically and effectively. It can be carried out in rural children and their living standard should be improved.

Biography

Speaker
Dr. AKM Mamunur Rashid /

Abstract

Ticks and tick borne parasites are a major constrain to good livestock productivity in the Sub-Saharan Africa including Nigeria. This study aimed at determining the prevalence of ticks and tick borne parasites in cattle at Shinge Livestock Market LafiaNasarawa State Nigeria. Ticks and blood collected from randomly selected cattle were examined using standard entomological, parasitological and haematological procedures. Out of the 200 cattle examined 112(56.0%) were tick infested. A total of 1,152 ticks were collected from all cattle examined. Rhipicephalus (Boophilus) decoloratus948(62.3%) was the most abundant species infesting cattle breeds followed by Amblyommavariegatum383(25.2%), then Hyalommarufipes 184(12.1%), while Rhipicephalussanguineus 7(0.5%) was the least tick species encountered. There was a very high significant difference (P < 0.00001) on species abundance. The bovine blood examined showed a 70.5% parasitic infection by two genera (Babesiaand Anaplasma).Babesiabigemina80(36.0%) was most prevalent, followed by Anaplasmamarginale77(34.7%), while Babesiabovis65(29.3%) was the least prevalent. There was no significant difference (P = 0.6848) in prevalence rate between tick borne parasites.Haematological investigation showed that blood indices examined were within standard ranges. It is therefore recommended that dips be introduced at strategic points to herders for easy access to de-tick their animals.

Biography

Faculty of Science, Federal University Lafia

Speaker
victoria Pam Adamu /

Abstract

Microbial approach is one of the best innovative tools for crops improvement. Achieving global food security in response to negative impact of environmental stresses and world population growth is the most challenging task for the 21st Century. The salinity and drought stresses are the major abiotic stress adversely affecting the plant productivity. Due to this the global food security is in danger, therefore, there is an urgent need to develop stress-tolerant plants with no yield loss under stress for future food security. Use of beneficial fungus (root endophyte Piriformospora indica) through non-transgenic and transgenic approaches could be one of the best innovative approaches for improvement plant productivity. Through non-transgenic approach P. indica has been found to provide strong growth-promoting activity during its symbiosis with a broad spectrum of plants including mustard, pea, tobacco, tomato, onions, rice, lepidium and many more medicinal plants. Despite its positive impact on the host, little is known about the P. indica genes that may be involved in stress tolerance to plants. To improve the plant productivity under salinity stress condition through transgenic approach, first high salinity-tolerant genes from P. indica need to be identified and cloned. We have cloned several salinity-tolerant genes from P. indica fungus by functional screening, based on random over-expression of a P. indica cDNA library in Escherichia coli grown on medium supplemented with 0.6 M NaCl. Out of these one of the salinity tolerant genes from P. indica has been functionally validated for its role in salinity tolerance in bacteria and plant both. This gene product functions as molecular chaperones and involved in pre-mRNA splicing. Overall, we demonstrated for the first time a direct evidence of countering salinity stress tolerance in plant by genetic modification using a fungal gene. Other approaches (plant to plant) for plant productivity improvement will be discussed, specially the emerging role of DNA and RNA helicases in stress tolerance. This research will be beneficial not only in enhancing the productivity of agriculturally important crops under adverse conditions but also will be helpful in increasing the income of the poor farmers, the most deserving for this.

Biography

An elected fellow of numerous national & international academies, Prof. Narendra Tuteja is currently Visiting Scientist at ICGEB, New Delhi. He is former Group Leader at ICGEB and Professor and Director at Amity Institute of Microbial Technology, Noida. He has made significant contributions to crop improvement under adverse conditions through genetic engineering and microbial approaches, reporting the first helicase from plant and human cells and demonstrating new roles of Ku autoantigen, nucleolin and eIF4A as DNA helicases. Furthermore, he discovered novel functions of helicases, G-proteins, CBL-CIPK, forisome, and LecRLK in plant stress tolerance, and PLC and MAP-kinase as effectors for Gα and Gβ G-proteins. Narendra Tuteja also reported several high salinity stress tolerant genes from plants and fungi and developed salt/drought tolerant crops including rice, groundnut, sugarcane, chili etc. Total publications: 375; Books: 20; Citaion <21,000; h-index: 56; i-10: 216

Speaker
Narendra Tuteja / Visiting Scientist & Former Group Leader, International Center for Genetic Engineering and Biotechnology

Abstract

The Pine Wilt Disease is a severe forest epidemic that has been devastating numerous pine forests, first in Asian countries, and then in European ones. Pinewood nematode, the pathogen of this disease, is native to North America and not pathogenic to its domestic pine species. This forest disease involves various biological relationships that may influence the disease's spreading manner. For instance, Monochamus beetles are the vector of the pathogenic nematodes and transfer the pathogen from dead trees to healthy ones. The fungal flora in the dead pine tree where the larvae of the vector beetle overwinter strongly influenced the number of pathogenic nematodes vectored by a beetle. Also, the host's resistance to this disease seems to be affected by the mycorrhizal relationship developing underground. The host-parasite relationship is the most important, of course. How could such a tiny nematode kill a huge pine tree so quickly? To understand this, we have to know the physiological host reactions against the pathogen, the conducting water system in a host stem, and the various traits of the pathogenic nematode evolved to parasitize pine trees.

Biography

will be updated soon

Speaker
Dr. Kazuyoshi Futai / An emeritus professor of Kyoto University

Abstract

Aminopeptidases are increasingly being investigated as therapeutic targets in various diseases. In this study, we cloned, expressed, and biochemically characterized a member of the methionine aminopeptidase (MAP) family from Babesiabovisto develop a potential molecular drug target. Recombinant B. bovis MAP (rBvMAP) was expressed in Escherichia colias a glutathione S-transferase (GST)-fusion protein, and we found that it was antigenic. An antiserum against the rBvMAPprotein was generated in mice, and then a native B. bovis MAPwas identified in B. bovisby a Western blot assay. Further, an immunolocalization assay showed that MAP is present in the cytoplasm of the B. bovismerozoite. Analysis of the biochemical properties of rBvMAP revealed that it was enzymatically active with optimum activity at pH 7.5 and stable at 37OC. Enhanced enzymatic activity was observed in the presence of divalent manganese cations and was effectively inhibited by a metal chelator, ethylenediaminetetraacetic acid (EDTA). Moreover, the enzymatic activity of BvMAP was inhibited by Amastatin and Bestatin as inhibitors of MAP in a dose-dependent manner. Importantly, MAPi were also found to significantly inhibit the growth of Babesia parasites in both in vitro and in vivo without any side effects; additionally, they induced immunopotentiating activities in the host. The findings of the present study suggest that BvMAP is a viable molecular drug target and that Amastatin and Bestatinmight be potential drug candidates for the treatment of babesiosis due to their dual antibabesial and immunopotentiating

Biography

Munkhjargal has completed her PhD from Obihiro University of Agriculture and Veterinary Medicine, Japan and postdoctoral studies from Obihiro University, Japan. She is a researcher of Institute of Veterinary Medicine, government organization. She has published more than 10 papers in reputed journals.

Speaker
Tserendorj Munkhjargal / National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Abstract

TiO2 nanoparticles incorporated GG film (2D) and scaffold (3D) was successfully fabricated. The fabricated 2D film and 3D scaffold was characterized using fourier transform infrared (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM) and termogravimetric/ differential thermal analyzer (TG-DTA) to study their physicochemical properties. The mechanical properties of film and scaffold was studied using an Instron Universal Testing machine (model 3366) with a load capacity ±10 kN grips and cross-speed set at 10 mm min-1. Water Vapour Transmission Rates (WVTR) and swelling was measured using American Society for Testing and Materials (ASTM) International Standard method. Meanwhile, the biological properties such as antibacterial of film and scaffold was observed qualitatively and quantitatively. Gram-positive (Staphylococcus aureus and Streptococcus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) microbes was for an anti-bacterial assay. In-vivo and in-vitro studies for biomedical application using fabricated 2D film and 3D scaffold was carried out. For in-vivo study, the excision wound model was applied on rats. An 8 mm wound was created using sterile wound bio-puncher. The measurement of the wound area was carried out from the day of the excision of the wound and at every three (3) days interval until the epithelisation of the wound was completed. On the other hand, in-vitro study of cell proliferation was quantified using a MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Commonly used cells are 3T3 mouse fibroblast, human skin fibroblast and etc.

Biography

Mohd Hasmizam Razali has a PhD degree in Materials Engineering (Nanomaterials) from Universiti Sains Malaysia (USM), MSc. in Chemistry (Catalyst) and B.Sc (Hons) in Chemical Industry from Universiti Teknologi Malaysia (UTM). Currently he is a Senior Lecturer at School of Fundamental Science, Universiti Malaysia Terengganu (UMT), Malaysia. He has published more than 50 technical papers in journals and conference proceedings locally and internationally related to the nanomaterials and functional materials research. He has been awarded Who’s Who in the World for 3 years in a row 2013, 2014 and 2015 by The Marquis Who’s Who Publications Board. In 2014, the Cambridge Biographical Centre listed him as one of 2000 Outstanding Intellectuals of the 21st Century, due its ability to produce nanomaterials with tremendous improvement compared to conventional commercial materials. On top of that, he is also the recipient of the MAWHIBA Award and GENEVA Gold Medal Award in 1999.

Speaker
Dr. Mohd Hasmizam Razali / Institute Name,School of Fundamental Sciences, Universiti Malaysia Terengganu

Abstract

Public health systems are not always prepared for outbreaks of infectious diseases. Although in the past several public health institutes, like the French ‘Institut Pasteur’ and the Dutch ‘Tropeninstituut‘, were prominent surveyors of infectious diseases, the investments in worldwide public health have decreased. Now more attention is given to curative healthcare compared to preventive healthcare. The recent Ebola Virus Disease outbreak in West Africa initiated a new wave of interest to invest in Worldwide Public Health to prevent outbreaks of highly contagious diseases. Zoonotic diseases are threatening as the population does not have natural nor artificial (from vaccination) immune response to new diseases like in the Ebola Virus Disease outbreak in 2014. The new strain of the Ebola Virus in West Africa was slightly less lethal, compared to other Ebola Virus strains, but the threat of spreading was far bigger as it had a longer incubation time. Most public health systems are not trained well enough to mitigate highly infectious and deadly disease outbreaks. NGO’s helping to fight the outbreak are often better trained in curative treatments and have less experience with biological (bioweapon) threats for which the military are trained for. The UNMEER mission was unique in this. It was a setting in which military and civilian actors cooperate in fighting a biological threat. Protection is essential for health workers. Smart systems have to be developed to prevent further spreading of the disease, but it is not only the biosafety, which has to be considered, but also the biosecurity, as misuse of extremely dangerous strains of microorganisms cannot be excluded. Several zoonotic infectious diseases, like anthrax, smallpox and hemorrhagic fevers are listed as potential bioweapons. Therefor both biosafety and biosecurity have to be implemented in all measures to fight

Biography

Works internationally for several medical and biotech companies as scientific advisory board member and is also an active reserve-officer of the Royal Dutch Navy in his rank as Commander (OF4). For the Dutch Armed Forces he is CBRNe specialist with focus on (micro)biological and chemical threats and medical- and environmental functional specialist within the 1st CMI (Civil Military Interaction) Battalion of the Dutch Armed Forces. For Expertise France he is now managing an EU CBRN CoE public health project in West Africa. He is visiting professor at the University of Rome Tor Vergata giving lectures for the CBRN Master study. In his civilian position he is at this moment developing with MT-Derm in Berlin (Germany) a novel interdermal vaccination technology as well as a new therapy for cutaneous leishmaniasis for which he has won a Canadian ‘Grand Challenge’ grant. With Hemanua in Dublin (Ireland) he has developed an innovative blood separation unit, which is also suitable to produce convalescent plasma for Ebola Virus Disease therapy. He has finished both his studies in Medicine and in Biochemistry in The Netherlands with a doctorate and has extensive practical experience in cell biology, immuno-haematology, infectiousdiseases, biodefense and transfusion medicine. His natural business acumen and negotiation competence helps to initiate new successful businesses, often generated from unexpected combinations

Speaker
Cdr Dr Stef Stienstra / Civil-Military-Interaction-Command Royal Dutch Armed Forces

Abstract

Finger millet is a small cereal grain with potential for use in commercial maltsby germinating its grains under controlled conditions, with the aim of activating endogenous hydrolytic enzymes, majorly α-amylase. The present study examined the effects of temperature and incubation time on α-amylase activity in finger millet seed. Moistened finger millet seeds were incubated at 15 ºC, 25 ºC or 35 ºC for five consecutive days. Samples were withdrawn daily at an interval of 24 h and kilned at 30 ºC and 40 ºC respectively. Radicles developments were monitored with measuring ruler, and amylase activity was determined with starch solution following standard protocols. The results revealed that radicle development of finger millet increased with germination time. However, seed germinated at 25 ºC and kilned at 30 ºC showed significant (P< 0.05) better radicle development than others germinated at 15 ºC and 35 ºC respectively. Similarly, seed germinated at 25 ºC and kilned at 30 ºC also showed the highest amylase activity than other counterparts. It is apparent that the optimal condition suitable for effective malting of finger millet was at 25ºC germination and kilned at 30ºC. For further study, finger millet malt will be fermented to produce clear beer or probiotic beverages.

Biography

Oluyinka Mary Amos-Awoniyi,is in the second year of her PhD in Microbiology at the University of Zululand, South Africa. She has published four papers in reputed journals while three of her research papers have been submitted for publications. She is serving as a reviewer and editorial board member for Asian Food Science Journal, CientPeriodique Journals (CPQ) and Acta Scientific Nutrition health Journal. Her Current research topic is “Alcoholic and lactic acid fermentation of finger millet and its potential for clear beer/ probiotics food beverage”. My area of research interestsareFood Microbiology,Food processing andNutrition.

Speaker
Ms. Oluyinka MAry Amos-Awoniyi / University of Zululand, South Africa.

Abstract

Echinococcus multilocularis is an important zoonotic parasite, responsible for alveolar echinococcosis in human beings. As an immune organ, the spleen plays a key role in host immune responses during E. multilocularis infection. Here we showed that the apoptotic levels of the spleen cells significantly decreased in mice 2 month and 3 month post infection of E. multilocularis (p< 0.05). To decipher the mechanism behind this, the miRNA profiles of these spleen cells were defined and found several miRNAs including miR-146a-5p were dysregulated. Bioinformatic analysis revealed that there were several potential miR-146a-5ptargets related to apoptosis including BAG-1, an antiapoptotic protein. Both miR-146a-5p and BAG-1 was validated to be down-regulated and up-regulated by PCRin mice 2 month and 3 month post infection of E. multilocularis, respectively.Moreover, BAG-1 was further validated to be up-regulated at the protein level by Western blotin mice 2 month and 3 month post infection of E. multilocularis. The results of dual fluorescent reporting assay verified that miR-146a-5p was able to bind to the 3’ UTR of BAG-1, suggesting that BAG-1 is its real target. In mouse spleen cells, miR-146a-5p over-expression led to a significant increase of apoptosis (p< 0.05), while miR-146a-5p low-expression induced a remarkable decrease of apoptosis (p< 0.001). Taken together these results indicate that downregulation of miR-146a-5p is attributed to a decreased apoptosis of the spleen cells via upregulating BAG-1, thus affecting immune responses against E. multilocularis.

Biography

Speaker
Yadong Zheng / Chinese Academy of Agricultural Sciences, Lanzhou, China

Abstract

Mutation Profile of pfdhfr and pfdhps in Plasmodium falciparum among Returned Chinese Migrant Workers from Africa Chao Xu, Hui Sun, Kun Yin Jin Li, Ting Xiao, Xiangli Kong, Yongbin Wang, Guihua Zhao, Ge Yan, Bingcheng Huang, Qingkuan Wei* Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, China We evaluated markers of sulfadoxine-pyrimethamine (SP) resistance in Plasmodium falciparum among 254 returned migrant workers in China from Africa from 2013 to 2016. High prevalences of pfdhfr (97.2%) and pfdhps (96.5%) mutations were observed. The partially resistant genotype was homogeneously distributed in Africa with a modestly high prevalence (48%), whereas the super resistant genotype was only found in West Africa with a very low frequency (1.2%). The findings provided baseline data about the molecular markers of SP resistance.

Biography

Biography Qingkuan Wei has completed his PhD from Jinan University, China. He research field is molecular mechanism of Toxoplasma immune protective and molecular epidemiology of malaria. He has published more than 15 papers in reputed journals, include Antimicrobial Agents and Chemotherapy, Vaccine and Scientific Reports.

Speaker
Qingkuan Wei / Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, China

Abstract

Surveillance of Antimalarial Resistance Pfcrt, Pfmdr1, and Pfkelch13 Polymorphisms in African Plasmodium falciparum imported to Shandong Province, China Chao Xu , Kun Yin, Qingkuan Wei, Hui Sun, Jin Li, Ting Xiao, Xiangli Kong, Yongbin Wang, Guihua Zhao, Song Zhu, Jingxuan Kou, Ge Yan, Bingcheng Huang* Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, China Antimalarial drug resistance is a major public health problem in China. From 2012 to 2015, more than 75% of malaria cases in Shandong Province were P. falciparum returned from Africa. However, molecular marker polymorphisms of drug resistance in imported P. falciparum cases have not been evaluated. In this study, we analyzed polymorphisms of the Pfcrt, Pfmdr1, and Pfkelch13 genes in 282 P. falciparum cases returned from Africa to Shandong between 2012 and 2015. Among the isolates, polymorphisms were detected in codons 74–76 of Pfcrt and 86, 184, 1246 of Pfmdr1, among which K76T (36.6%) and Y184F (60.7%) were the most prevalent, respectively. Six Pfcrt haplotypes and 11 Pfmdr1 haplotypes were identified and a comparison was made on the prevalence of haplotypes among East Africa, West Africa, Central Africa and South Africa. One synonymous and 9 nonsynonymous mutations in Pfkelch13 were detected in the isolates (4.6%), among which a candidate artemisinin (ART) resistance mutation P553L was observed. The study establishes fundamental data for detection of chloroquine resistance (CQR) and ART resistance with molecular markers of the imported P. falciparum in China, and it also enriches the genetic data of antimalarial resistance for the malaria endemic countries in Africa.

Biography

Biography Chao Xu has completed his PhD from Jinan University, China. He research field is polymorphism of plasmodium falciparum resistant markers and epidemlology and surveillance. He has published more than 10 papers in reputed journals, include Antimicrobial Agents and Chemotherapy and Scientific Reports.

Speaker
Chao Xu / Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, China

Abstract

Prediction of Toxoplasma gondii virulence factor ROP18 competitive inhibitors by virtual screening Guihua Zhao, Chao Xu, Qingkuan Wei, Bingcheng Huang, Kun Yin * Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences Rhoptry protein 18 (ROP18) is a key virulence factor of Toxoplasma gondii. The host’s IRGs could be blocked by it. ROP18 also interacts with ATF6β and affects multiple physiological functions within host cells, thereby inducing intense virulence. In this study, competitive inhibitors targeted to ROP18 were subjected to virtual screening based on the principle of structure-based drug design. The preparation of the ROP18 structure was conducted using Molecular Operating Environment software. The ATP-binding pocket was selected as the starting point. Construction of the pharmacophore model used EHT half-quantitative measurement. The pharmacophore model of ROP18 was imported into the Specs database for screening. Hit compounds were selected using London dG and GBVI/WSA scoring. The top 100 hits were analyzed by molecular docking and structure activity relationships (SAR) analysis. The final pharmacophore comprised three typical characteristics: three hydrogen bond acceptors/donors, two ring aromatic features occupying the hydrophobic core, and one cation group feature targeted to the terminus of ATP. A total of 1314 hits analogous to ROP18 pharmacophore were passed through the Specs. After two rounds of docking, 25 out of 100 hits were identified as belonging to two main scaffold: phthalimide ring structure, thiazole ring and styrene structure. The screen also identified 13 inhibitors with distinct scaffold. These hits could engage in multiple hydrogen bonds, salt bridges halogen bonds, and hydrophobic interactions with ROP18, and para-position halo substituents on the benzene ring may enhance their affinity scoring. Our results provide useful scaffold types for the chemical inhibition of ROP18 or alternative conformations to develop new anti-toxoplasmosis drug leads.

Biography

Biography Kun Yin, female, born in 1980, associate professor and master supervisor. Kun Yin has completed her PhD from Shandong University, China. Research interests: ① Structure of novel vaccine/drug target candidates; ② Interaction factors and mechanism of pathogens such as Toxoplasma gondii and Legionella pneumophlia. Headed over 10 research funding projects including the National Natural Science Foundation, the Natural Science Foundation of Shandong Province and the Medicine and Health Science Technology Development Plan of Shandong Province. Won a total of 9 Science and technology achievement awards. She has published more than 58 papers and has a national invention patent at present.

Speaker
Kun Yin / Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences

Abstract

Purification and characterization of Toxoplasma gondii immune mapped protein-1 Kun Yin, Chao Xu, Guihua Zhao, Qingkuan Wei, Bingcheng Huang, Ge Yan* Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences To express and purify the Toxoplasma gondii RH strain immune mapped protein 1 (IMP1) protein through the prokaryotic expression system, and to identify the purified protein. Gene of TgIMP1 was attained by RT-PCR and the amplified product was identified by TA-clone and sequencing. The identified fragment of IMP1 was connected into the expression vector pET28b and the recombinant pET28b-TgIMP1 was identified by double digestion and sequencing. The fusion protein TgIMP1 with 6×His tag was expressed in E.coli BL21 (DE3) host and the optimal inducing conditions were investigated by adjusting the harvesting time interval, inducing temperature and concentration of isopropyl β-D-thiogalactoside (IPTG). The solubility detection of TgIMP1 protein and the massive purification of it were conducted with Ni2+-affinity purification. The purified product was identified by Coomassie-stained SDS-PAGE and immune-blotting. The prokaryotic expression recombinant pET28b-TgIMP1 was successfully established on the basis of the identified TgIMP1 gene by TA-clone. Recombinant proteins of TgIMP1 could be efficaciously and largely expressed in E.coli BL21 strain with 0.3 mM IPTG for 9 h at 20°C, and with the characteristics of good stability, high solubility and purity, and fine immunity. TgIMP1 protein could be expressed in the prokaryotic host strain with satisfied stability and solubility. Our findings may lay the foundation for the future protein-based investigations such as development of subunit vaccine and structural determination.

Biography

Biography Ge Yan has completed his PhD fromChongqing Medical University, China and postdoctoral studies from Hongkong University, China. He is the director of pathogeny biology in Shandong Institute of Parasitic Disease. He has published more than 40 papers in reputed journals and has been serving as an editorial board member of repute.

Speaker
Ge Yan / Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences

Sessions:

Microbiology and Parasitology

Abstract

Companion animals (dogs, cats) and food animals (cattle, swine) are subject to bacterial infectious diseases similar to what occurs in humans. For example respiratory infections occur in cattle and swine and respiratory, urinary and skin and skin structure infections (SSTI) occur in dogs and cats. For urinary tract infections, E. coli is a predominant pathogen in humans and animals and other organisms such as Pseudomonas aeruginosa and Proteus mirabilis are also in common. For SSTI, Staphylococcus aureus is a common human pathogen as compared to Staphylococcus pseudintermedius in dogs and these 2 species share many common virulence factors including resistance to methicillin by a novelmecA gene. Advances in technology for organism identification is thought to be one reason why animal pathogens seem to be more frequently identified from human specimens over the past 5 years in our hospital. Are such organisms clinically significant? In reviewing the clinical findings of patients from which S. pseudintermedius was recovered, 1 pediatric patient had bacteremia, 1 adult patient had multiple specimens over a 45 day period from which the organism was recovered, including a colonized indwelling abdominal catheter and several patients had SSTIs including diabetic patients with “diabetic foot” related infections. Further investigation of family pets from some of these patients resulted in identical strains recovered between pet and owner but different between patients suggesting multiple transmission events from pet to owner and no one predominant strain. Antibiotic therapy was warranted in these cases. Recovery of animal pathogens from human specimens require careful consideration with the patients clinical findings.

Biography

JM Blondeau completed his Ph.D from the University of Manitoba, Winnipeg, Canada and his post-doctoral training in Clinical Microbiology at the Victoria General Hospital and Dalhousie University, Halifax, Canada. He is Head of Clinical Microbiology at Royal University and the Provincial Lead for Clinical Microbiology in Saskatoon, Canada. He has published ~175 papers, 240 abstracts and 5 books. He has given more than 640 lectures worldwide in 44 countries. He has been twice nominated for a University of Saskatchewan Student Union Teacher of the Year Award. He is the current Editor-in-Chief of Expert Reviews in Respiratory Medicine

Speaker
Joseph Blondeau / 1Royal University Hospital, Saskatchewan Health Authority and the 2University of Saskatchewan, Saskatoon, Canada

Abstract

Background: Warung Tenda (Indonesian Traditional Food Stalls) is a typical street food widely spread in Indonesia and globally distributed with many forms of diversity. Warung Tenda serving raw vegetables with minimal food processing. Personal, handling and Warung Tenda's hygiene also determine the viability of parasites contamination. Objectives: To evaluate parasite contamination in raw vegetables and hygiene aspects in Warung Tenda among Palu City, Indonesia. Methods: Cross-sectional study was carried out among 86 participants in Palu City, Indonesia in March and April 2019. Warung Tenda's sampling was done by purposive sampling. 4 different vegetable species of Cabbage (Brassica oleraceavar capitate L), Holy basil (Ocimum sanctum L), Cowpea (Vignasinensis L), and Cucumber (Cucumissativus L) was tested for parasites. Result: The study found that T.gondii was the most prevalent parasite 15 (17.4%), followed A.lumbricoides 8 (9.3%), Hookworm 3 (3.5%), T.trichiura and E.histolytica 1 (1.2%). Parasite prevalence was higher in Holy Basil, Cabbage Cowpea, and Cucumber (17.4%; 9.5%; 3.36%; 3.36% respectively). Personal hygiene mostly with medium quality compared with good and lack (55.8% vs 20.9%). Handling hygiene was higher in medium quality, good, and lack (55.8%; 29.1%; 15.1%). Warungtenda’s hygiene most prevalent with good quality, moderate and lack (69.8%; 27.9%; 2.3%). Conclusion: Non-GI parasites were more prevalent than GI parasites. Even though mostly Warung Tenda in Palu had a good quality for place setting; Sold raw vegetables still need improvement in handling processing and handler hygiene to eliminate parasite existence.

Biography

Salim is undergraduate medical student in Alkhairaat University Palu City, Indonesia. He is very interesting in parasitology and public health and highly motivated to do this research eventhough there were some rejection of the food stall’s owner in the beginning of his research. He also had some difficult when the earthquake happened in Palu on September 2018 that many of the Warung Tenda were closed after that earthquake. Fortunately after several months Palu have had recovered from this situation.

Speaker
Mr. Salim / 1. Medical Student, Medical Faculty, Alkhairaat University, Palu City, Central Sulawesi, Indonesia

Abstract

Background: Quinolones are one of the most important classes of antibacterial available for the treatment of infectious diseases in humans. However, the increasing clinical utility of fluoroquinolone in treatment of many infections including urinary tract infection (UTI) has been increasing the number of resistant bacterial strains. The most common and significant form of resistance is caused by specific mutations in gyr A (gyrase) and pariCi(topoisomeraseiIV)genes.uThesemutations conferring resistance to fluoroquinolones (FQs) arise within the quinolone resistance determine region (QRDR) in chromosomalgyr A and par C subunits. The aim: the study designed to determine the relation between wild and mutated of gyr A gene at codon and parC gene at codon that may provide the molecular explanation for resistance of clinical uopathogenicE. coli (UPEC) isolates that isolated from Libyan UTI patients. Material and methods: total of 61 UPEC isolates were collected from the microbiology laboratory, Tripoli Medical Center Laboratory (TMC) and Reference Medical Central Laboratory (RMC) between the period from November 2013 to April 2014. Identification and antibiotic susceptibility of UPEC isolates were performed using Vitek 2 compact automated system. Results: Fluoroquinolone resistance rates to UPEC isolates were 39%, 35% and 34% tociprofloxacin, levofloxacin and moxifloxacin, respectively. While the high resistance rates were detected in ampicillin (88%) and piperacillin (84%). MDR was observed in 56% isolates. Fluoroquinolone resistance rate among ESBL uropathogenicE. coli was 64%.Polymerase Chain Reaction (PCR) amplification and sequencing of the QRDR of gyr A and par C were performed to identify the mutations for 29 UPEC isolates. Several variations were identified in gyrAand parCgenes were in both susceptible and resistant isolates, whereasthe most wild type E. coli isolated were ciprofloxacine resistanceand double mutation were predominant in mutant E. coli isolated. The amino acid substitutions of UPEC isolates in QRDRs of gyr Agene were the serine at position 83 altered to leucine, phenylalanine and proline, whereas the aspartic acid at position 87 altered to asparagine, valine and lysine. While the amino acid substitutions in parC were found the serine at position 80 altered to isoleucine, tyrosine and arginine, whereas the glutamic acid at position 84 changed to valine, aspartic acid, alanine and arginine. The nucleotide sequencing analysis were identified into 4 distinct patterns in gyr A gene and 6 distinct patterns in pariCgenes. The patterns of alteration in gyriA among ciprofloxacin resistance UPEC isolates were found one single mutation (Ser83Leu), and one double mutation (Ser83Leu+Asp87Asn), while the patterns of alterations in pariCwereonesinglemutation (Ser80Ile) and one double mutation (Ser80Ile+Glu84Val). In addition, the most common mutation noticed in ciprofloxacin resistance isolates were at Ser83Leu and Asp87Asn of gyriA and Ser80Ile of par C genes. Conclusion: the study confirms the presence of mutations responsible for fluoroquinolone resistance in QRDR of gyriAand pariCgenes of UPECisolates.

Biography

Speaker
Dr. Ali Daeki / The Libyan Academy of Graduate Studies, Tripoli-Libya

Abstract

Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. PCR, although very sensitive and specific, has limited utility in malaria endemic areas because of complex methodologies, high cost, and the need for trained technicians and special facilities. Therefore, microscopic examination of the Giemsa stained blood smears remains the gold standard for the diagnosis of malaria even though it has limited sensitivity and specificity in field conditions. We have developed three Plasmodium FISH (Fluorescent in Situ Hybridization)assays, that are simple, rapid and detect all stages of the parasites. The Plasmodium Genus (P-Genus) FISH assay detects all five species of Plasmodium known to cause the disease in humans. The P. falciparum (PF) - P. vivax (PV) combo FISH assay detect and differentiate between P. falciparum and P. vivax. The P. knowlesi (PK) FISH assay detects and differentiate P. knowlesi from other Plasmodium species. The FISH assays are reproducible and have sensitivity and specificity, which is superior to that of the widely used microscopic examination of Giemsa stained blood smears. The only requirement is anincubator and a LED unit with blue-green filter that can be attached to a regular light microscope with 100X objective to read FISH processed smears. Major advantages of FISH assays are: (1) detectslive parasites, (2) provides morphology and (3) speciation based on specific rRNA sequence whereas, Giemsa results are based on morphology only.

Biography

Dr. Jyotsna Shah is the President and Laboratory Director of IGeneX Clinical Laboratory, Milpitas, CA and Vice President at ID-FISH Technology Inc., Milpitas, CA. Dr. Shah has over 40 years of research experience in immunology, molecular biology and microbiology. She got her B.Sc. and M.Sc in Biological Sciences from UK and her Ph.D. in diagnostic immunology from Nairobi University, Kenya. She then joined International Laboratory for Research on Animal Diseases (ILRAD) as a post-doctoral scientist where she started the first DNA sequencing laboratory in E. Africa. On completion of her fellowship, she joined Harvard University, Department of Tropical Medicine, Boston as a research fellow and continued work on development of molecular tools for diagnosis of parasitic diseases. Since then she has worked at several Biotechnology companies, mostly involved in development of novel molecular technologies for diagnosis of infectious diseases. She is a world expert on use of Fluorescent in Situ Hybridization (FISH) technique for direct detection of pathogens in clinical samples. She has many publications and over 20 patents. She joined IGeneX as the Director of Research and Development in 1997. She introduced the first Fluorescent In Situ Hybridization (FISH) test for Babesia and also set up the PCR department for tick-borne diseases. Since April 2003 she has been the Laboratory Director (clinical and research). She represents IGeneX at local and international meetings. Under her guidance, the laboratory has developed an excellent QA program. Because of her scientific guidance IGeneX has become one of the leading reference laboratory for diagnosis of tick-borne diseases in the world.

Speaker
Dr. Jyotsna Shah / ID-FISH Technology Inc., Milpitas, CA 95035, USA

Abstract

In light of the shift to aiming for schistosomiasis elimination,we determined prevalence, reinfections, participation, sample submission adherence, and effect of treatment on schistosomiasis prevalence in children aged 5 years and below in an endemic district in Zimbabwe, over one year.The study was conducted from February 2016‒February 2017 in Madziwa area, Shamva district. Following community mobilisation, mothers brought their children aged 5 years and below for recruitment at baseline and urine sample collection at baseline, 3, 6, 9 and 12 months follow up surveys. At each time point, urine was tested for urogenital schistosomiasis by urine filtration and children found positive received treatment. Of the 535 children recruited from the five communities, 169 (31.6%) participated consecutively at all survey points. The highest mean number of samples submitted was 2.9 among communities and survey points. S. haematobium prevalence significantly reduced from 13.3% at baseline to 2.8% at 12 months for all participants and from 24.9% at baseline to 1.8% at 12 months (P< 0.001) for participants coming at all time points. Reinfections were significantly high at 9 months follow up survey (P = 0.021) and in Mupfure (P =0.003). Logistic regression analysis showed that the risk of acquiring schistosomiasis was high in some communities (P< 0.05). S. haematobium infections and reinfections are seasonal and depend on micro-geographical settings. Participation compliance at consecutive visits, sample submission adherence,and sustained treatment of infected individuals are important for effective operational control interventions.

Biography

Speaker
Mrs. Masceline mutsaka-makuvaza / 1University of Zimbabwe, College of Health Sciences, Department of Medical Microbiology.

Abstract

Introduction: Toxoplasma gondii, the etiological agent of toxoplasmosis, can cause severe or lethal damages in both animals and man. So, the development of a more effective vaccine to prevent this disease is extremely needed. The dense granule antigen 14 (GRA14) has been identified as a potential vaccine candidate against T. gondiiinfection. Methods: In this study, we evaluatedprotective immunity induced by prime/booste regimes of GRA14 antigen with or without calcium phosphate (CaNPs) and Aluminum hydroxide (Alum)nano-adjuvants in BALB/c mice.Likewise,BALB/c mice were immunized three times according to specific immunization schedules andthe production of IgG antibodies, lymphocyte proliferation, and level of gamma interferon (IFN-γ) and interleukin 4 (IL-4)were detected after vaccination.In addition, the survival rate and parasite burden of mice challenged with1 × 104tachyzoites of T. gondii RH strain were evaluated. Results: The finding showed that immunization with the prime-boost strategy using plasmid DNA (pcGRA14) and recombinat protein (rGRA14) with nano-adjuvants significantly elicited levels of specific IgG antibodies and cytokines against T.gondii infection.Given that, there were the high levels of total IgG, IgG2a isotype, IFN-γ, and specific lymphoproliferative responses in mice of groups rGRA14+ CaNPs and pcGRA14+ rGRA14-CaNPs, which indicating a Th-1 type response. While immunization of mice with Alum based rGRA14 and pcGRA14+ rGRA14 elicited specific IgG1 and IL-4 levels, which was confirmed a Th-2 type response.In addition,mice immunized with prime/booste regime with calcium phosphate nano-adjuvant significantly prolonged the survival time and decreased parasite burden in brain tissue compared to the other groups (P<0.05). Conlusion: The vaccination studyshowed that the DNA priming and recombinant protein boost strategy is a promising approach to induce protective immunity against T. gondii infection.

Biography

Speaker
Dr. Ahmad Daryani / Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran

Abstract

Introduction: The persistence of resistance Plasmodium falciparum strains and the treatment failure related to conventional antimalarial has led to substitution of these antimalarial by Artemisinin-based combination therapies (ACTs) for treatment uncomplicated malaria and Sulfadoxine-Pyrimethamine (SP) for intermittent preventive treatment (IPT-SP) in women pregnancies in Gabon (WHO, 2005). Studies showed that chloroquine resistance may revert to sensitivity after its withdrawal mainly detected by a significant decrease of Plasmodium falciparum Pfcrt and Pfmdr1 alleles. Quintuple mutation Pfdhfr (51I-59R-108G)+Pfdhps-437G-540E/581 alleles is predictive of treatment failures to SP. A genome-wide association study reported recently that non synonymous polymorphisms in Pffd-D193Y, Pfarps10-V127M, Pfmdr2-T484I, Pfcrt-326S and Pfcrt-I356T were also associated extension of time of clearance parasite or in clinical failures at ACTs. These molecular markers were analyzed in P.falciparum isolates collected from patient in Melen. Patients and Methods: Cross-sectional study was conducted between 2014 in the Regional Hospital of Melen (RHEM) locate in north of Libreville. Blood samples were collected from febrile patients enrolled from clinical trial. Giemsa-stained thick and thin blood smears of the peripheral blood were performed in each patient for malaria diagnostic. DNA samples of day 0 (before treatment) were extracted after leukocyte-depleted and sequenced. Results: A total, 37 isolates of patients randomized on 58 isolates analyzed. None influence parasites genotypes on treatment outcome of patients was found. The frequency of mutant CVIET (IET) allele of Pfcrt gene was detected in 75.8% (n=44/58) of isolates. The wild-type N86-F184-D1246 (43.1% ; 25/58) haplotype of Pfmdr1 gene was more frequently. The quintuple mutation Pfdhfr (51I-59R-108N)+Pfdhps-437G-540E was detected. Indeed, 94.5% (35/37) of patients were infected by haplotype VDNT-Pfmdr2-484I. Overall, all the patients were infected by the parasites carrying mutant Pfcrt-356T allele while the mutant Pfmdr2-484I allele was detected in 30.7% (n=16/52) of isolates. None mutant Pfarps10-127M, Pffd-193Y and Pfcrt-326S allele were found. The frequency of wild Pfmdr2-484T allele was 3 fold higher compared mutant Pfmdr2-484I allele. The median density parasite was lower in the patients infected by parasites carrier of VDNTI+ Pfcrt-CVMNK (H0) et VDNTI/CVIET (H1) haplotypes while it was higher those infected by haplotype VDNTT+ Pfcrt-CVIET (H2), VDNT*+Pfcrt-CVIET/CVMNK (H3) and VDNTT+ Pfcrt-CVIET/CVMNK (H4). Conclusion: The carriage of multiple mutations no influence on treatment outcome. However, a relationship between Pfmdr2-484I + Pfcrt-CVMNK haplotype was associated with the presence of parasite density low and the Pfmdr2-484T + CEVIET haplotype with parasite density high.

Biography

Speaker
Dr. Jacques Mari Ndong Ngomo / Faculty of Medicine, Department of Parasitology and Mycology, Université des Sciences de la Santé, BP 4009, Owendo, Gabon

Abstract

The most common and pathogenic bird’s nematode, Ascaridia has about 49 species, and only morphology-based species identification is really challenging. There are a few molecular data of Ascaridia species in GenBank. The nematodes were collected from the small intestines of domestic chickens in Bangladesh and analyzed on their morphological and molecular characteristics. The nematodes were morphologically identified as Ascaridia galli. The ITS1 region sequences (686bp) of the nematodes had intraspecific variation (0 – 0.27%) and were 99.70 – 100% similarities with those of A. galli (KX683286) and Ascaridia columbae (JQ995321) which is morphologically similar to A. galli and parasitze pigeon, and were 84.80% and 76.47% similarity with those of Ascaridia compar (FM177755) and Ascaridia nymphii (MF375321), respectively. The ITS2 region sequences (502 – 513bp) had intraspecific variation (0 – 1.76%), and were 99.70 - 100% similarities with those of A. galli (KX683286 and KY789472 etc), and were 97.73 – 99.13% and 51.01% similarity with A. columbae (JQ995321) and A. nymphii (MF375321), respectively. The cox1 sequences (483 bp) showed 24 haplotypes with 0.19 – 2.30% variations and high variation (16.60 – 18.30%) with A. columbae. The cox1 haplotypes of A. galli from Bangladesh formed some clusters along with those from South Africa, China, Denmark and Brazil in the phylogram. The lack of molecular data hampers our efforts in studying phylogenetic relation with the other Ascaridia species. Our molecular data will be used for DNA barcoding and phylogenetic analyses in future. Further molecular data of the other Ascaridia species are needed for elucidating molecular identification and phylogenetic relation.

Biography

I am third year PhD student in Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Japan under the Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Japan. I finished my DVM and MS in Parasitology from Bangladesh Agricultural University, Mymensingh, Bangladesh. I worked on Food and Agriculture Organization of United Nation in Bangladesh as a Veterinarian for active surveillance on Avian Influenza. Before joining the PhD course I worked as food safety Veterinarian on Khulna City Corporation, Bangladesh. I have three published paper till now.

Speaker
Mr. Peru Gopal Biswas / 1. Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

Abstract

Intestinal parasites are the major reason for morbidity and deaths all over the world especially in the third world. Poverty, lack of education, poor sanitation, unclean drinking water, hot and moist environment are the conditions responsible for these parasitic ailments. Poor personal hygiene among children is considered an effective cause of parasitic invasion. In present study the prevalence of intestinal parasites and their associated risk factors were determined among the primary school children of Lahore, Pakistan. A total of 150 faecal samples were collected from the children of 3-15 years of age belonging to Private and Government Schools. Different techniques like Direct smear method, Formalin ether concentration technique, Sedimentation technique and McMaster were used to identify different stages of intestinal parasites. Different stages of parasites were identified by these techniques. The parasites found were Giardia lamblia (4.66%), Entamoebahistolytica (3.3%), Ascarislumbricoides (4.66%), Taeniasaginata (4%), Hymenolepis nana (2%), Trichuristrichura (2.66%) and Enterobiusvermicularis (4%). A. lumbricoides was found to the most frequent of all parasites. The prevalence was found to be more among the individuals, with poor hygiene, having lack of education especially of mothers because they play a big role in child’s upbringing and maintaining his good health. Educating cleanness alertness on parasitic diseases and application of helpful strategies for parents to raise socioeconomic circumstances may decrease the load of infection.

Biography

Speaker
Dr. Asma Abdul Latif / Department of Zoology, Lahore College for women university, Lahore

Abstract

Amebiasis is an intestinal disease transmitted by the protist parasite, Entamoeba histolytica, following the ingestion of contaminated food and water. Many infected patients (90%) are asymptomatic but for unknown reasons, these trophozoites can become virulent and invasive, cause amebic dysentery, and migrate to the liver, via the portal veins, where they cause hepatocellular damage. We have recently reported that Escherichia coli can modulate E.histolytica 's virulence and resistance to oxidative stress (OS) via the production of oxaloacetate. Queusine is a naturally occurring modified nucleoside and is found in the first position of the anticodon of the transfer RNAs for asp, asn, his and tyr. Lower and higher eukaryotes are supplied with queuine (the base of queuosine) in the diet or by the intestinal flora. Here, we have tested the hypothesis that queuine is one of the trigger(s) that switches non-virulent E.histolytica into a virulent parasite. Queuine which is efficiently incorporated in E.histolytica’s tRNAs leads to significant modifications of the parasite’s transcriptome, to hypermethylation of C38 in tRNAAsp-GTC and to OS resistance. We have biochemically characterized the parasite’s tRNA-guanine transglycosylase (TGT), the enzyme that catalyzes the queuosine modification of tRNAs. Trophozoites silenced for TGT expression are poorly incorporating queuine in their tRNA and they are less resistant to OS compared to control trophozoites.

Biography

Serge Ankri has completed his PhD from The Faculty of Paris 11, France and postdoctoral studies from the Weizmann Institute, Israel. He is the head of the Molecular Parasitology Laboratory at the Faculty of Medicine, Technion, Israel. He has published more than 60 papers in reputed journals and has been serving as an editorial board member of repute journals.

Speaker
Dr. Ankri Serge / Department of Molecular Microbiology, Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel

Will be updated soon...