Scientific Program

Keynote Talks

Abstract

"Thoughts on the Life Periods of our Earth Bela Ralovich Ministry of Welfare (Retired), Budapest, Hungary Abstract The author thinks that the endless and timeless Universe is a permanent substance and energy flow as well as motion of celestial bodies. Celestial bodies may have physical and biological life. Our Earth has both lives which can be divided into different periods of time. Its physical life can be characterized by the atomic evolution, sterility and appearance of water. The essence of the biological life is the existence of living organism. The biological life of our Globe has started with appearance of the first microorganism influencing on its environment which is a closed system for substance, flora, creature and man but not for hit of meteors and for arrival as well as living of energies. The biological life our Earth can be divided into four parts: before photosynthesis, before man kind, before 1778 and after industrial revolution."

Biography

Ralovich Bela was born in 1937 at Pecs (Hungary). He received his M.D. diploma in the Medical University of Pecs. He started to work for the Institute of Microbiology (1961-1975) near professor Rauss and latter for the Institute of the Public Health and Epidemiology (1975-1988). He thought medical students and his main research fields were: enteric bacteria, salmonellosis, salmonella immunity, listeriosis, food microbiology and microbial flora of surface waters. He performed researches in the U.K. as well as in Russia and presented scientific lectures in 14 countries. He was a WHO expert for listeriosis and organized two International Listeriosis Symposia. He received Ph.D. (salmonellosis) in 1973 and D.M.S. (listeriosis) in 1986 from the Hungarian Academy of Sciences (HAS). In 1988 he became an expert in the Research Institute of the Hungarian Meat Indistry (1988-1992) and finally a senior executive of public health in the Ministry of Welfare (1992-1999) at Budapest. In 1999 he retired. He published 4 books, more lecture notes, some chapters and more than 165 articles. He was the member of several Hungarian and International (American, British, Taxonomical, Food Microbiological and so on) Societies and is an active member of the Hungarian Society for Microbiology still. His scientific activity was recognised with different awards (Markusovszky Diploma, Outstanding Scholar Award of the HAS (three times), CDC Medal (USA), Manninger Medal and the Golden Cross of the Hungarian Republic).

Speaker
Ralovich Bela / Ministry of Welfare (retired), , Hungary

Abstract

Premeltons are examples of emergent structures (i.e., structural solitons) that arise spontaneously in DNA due to the presence of nonlinear excitations in its structure. They are of two kinds: B-B (or A-A) premeltons form at specific DNA-regions to nucleate site-specific DNA melting. These are stationary and, being globally nontopological, undergo breather motions that allow drugs and dyes to intercalate into DNA. B-A (or A-B) premeltons, on the other hand, are mobile, and being globally topological, act as phase-boundaries transforming Binto A DNA during the structural phase-transition. They are not expected to undergo breather-motions. A key feature of both types of premeltons is the presence of an intermediate structural-form in their central regions (proposed as being a transition-state intermediate in DNA-melting and in the B- to A- transition), which differs from either Aor B- DNA. Called beta-DNA, this is both metastable and hyperflexible and contains an alternating sugar-puckering pattern along the polymer-backbone combined with the partial-unstacking (in its lower energy-forms) of every other base-pair. Beta-DNA is connected to either B- or to A- DNA on either side by boundaries possessing a gradation of nonlinear structural-change, these being called the kink and the antikink regions. The presence of premeltons in DNA leads to a unifying theory to understand much of DNA physical-chemistry and molecular-biology. In particular, premeltons are predicted to define the 5 and 3 ends of genes in naked-DNA and DNA in activechromatin, this having important implications for understanding physical aspects of the initiation, elongation and termination of RNA-synthesis during transcription. For these and other reasons, the model will be of broader interest to the general audience working in these areas. The model explains a wide variety of data, and carries within it a number of experimental predictions all readily testable as will be described in my talk.

Biography

Henry M. Sobell completed his studies at Brooklyn Technical High School (1948- 1952), Columbia College (1952-1956), and the University of Virginia School of Medicine (1956-1960). Instead of practicing clinical medicine, he then went to the Massachusetts Institute of Technology (MIT) to join Professor Alexander Rich in the Department of Biology (1960-1965), where, as a Helen Hay Whitney Postdoctoral Fellow, he learned the technique of single crystal X-ray analysis. He then joined the Chemistry Department at the University of Rochester, having been subsequently jointly appointed to both the Chemistry and Molecular Biophysics departments (the latter at the University of Rochester School of Medicine and Dentistry), becoming a full tenured Professor in both departments (1965-1993). He is now retired and living in the Adirondacks in New York, USA.

Speaker
Henry M. Sobell / University of Rochester, USA

Abstract

Microbial resistance to antibiotics is a worldwide problem in human and veterinary medicine. The epidemiology of antimicrobial resistant microorganisms at the human animal interface involves complex and largely unpredictable systems that include transmission routes of resistant bacteria as well as resistance genes and the impact of antimicrobial selective pressures in several reservoirs (animals, humans, and the environment. Typically, wildlife is not exposed to clinically use antimicrobial agents but can acquire antimicrobial resistant bacteria through contact with humans, domesticated animals and the environment, where water polluted with faeces appears to be the most significant vector of contamination. The incidence of commensal and pathogenic bacteria in faecal contaminations can be expected to be a connection between the environment and settings with regular or even constant antimicrobial pressure (aquaculture, livestock farming, human, and veterinary clinical settings), resulting in a unceasing release of antibiotic-resistant human and animal bacteria into the environment through wastewater or manure. The feature that particularly differentiates antimicrobial resistance from other food safety related problems is the role of the chemical driver, the antimicrobials, which selects for the resistant bacteria that subsequently can spread between animals and humans.Transmission of resistance from animals to humans can take place through a variety of routes where the food-borne route probably is the most important (most infections with enteric bacterial pathogens, such as Salmonella enterica, Campylobacter coli/jejuni, and Yersinia enterocolitica, probably occur through this route in industrialized countries), whereas, for other resistant pathogens, direct contact between animal and humans may be the major route of transmission (e.g., MRSA CC398). Bacteria as well as antibiotic residues from food-animal production are spread widely in the environment, mainly with manure, where it affects bacteria in the environment as well as in wild fauna. Thus, the environment and wild fauna can become reservoirs of resistance and a source of reintroduction of resistant bacteria into the food-animal and human reservoirs.

Biography

Patricia Poeta holds a PhD in Veterinary Medicine by the University of Tras-os-Montes e Alto Douro (UTAD), Vila Real, Portugal, and she is a specialist in Medical Microbiology. Actually, she is Full Professor of Medical Microbiology of the Department of Veterinary Science at UTAD, coordinator of the Research Group of Antibiotic resistance and head of Medical Microbiology Laboratory. Her main research interest is the characterization of antibiotic resistance mechanisms in bacteria of different origins as humans, animals, food, water and the environment, as well as the performance of studies of molecular epidemiology to characterize the genetic lineages of antibiotic resistant bacteria. Member of the Centre for Studies in Animal Science and Veterinary (CECAV). Director of 9 doctoral and 21 masters thesis. Responsible for research projects funded by private companies and inter-university projects, teaches International courses and Masters degree and keeps working activities with several national and international entities. With regard to scientific production she has published more than 100 articles in journals from the SCI, 7 book chapters, more than 160 communications at national and international scientific meetings, 37 presentations at national or Iberian scientific meetings, 6 publications in GenBank, 7 publications in UniProt, 6 records in MLST, and 43 technical and scientific journals and series or educational work. Received 16 awards. Operates in the areas of Veterinary Science. In professional activities interacted with 155 workers in authorship and co-authorship of scientific papers.

Speaker
Patricia Alexandra Curado Quintas Dinis Poeta / University of Tras-os-Montes e Alto Douro, Portugal

Sessions:

Microbiology

Abstract

In parasitized sheep, reductions in immunity to fight against endo-parasites might be attributable to mineral deficiencies. Trace elements including Cu, Co, Mn and Zn were determined in sera of sheep, preferred flora and respective soil samples. An associative analysis was made between the serum concentrations of the study elements and quantitative worm burden. A total of 384 blood and faecal samples from grazing sheep, forages and soil samples were collected fromSialkot district. Overall prevalence of GI parasites was 32.55%. Variable age and breed showed non-significant results while, sex was found significant. Concentration of selected trace elements was significantly (P > 0.05) varied in analyzed forages. A non-significant (P > 0.05) variation among soil trace elements was found in different tehsils of Sialkot district. Concentration of Zn in serum showed significant (P < 0.05) results among tehsils of district Sialkot. Mean concentrations of Zn, Co and Cu in serum was found inversely proportional to the mean egg per gram of sheep. Forages containing high concentrations of Zn, Cu and Co can be used effectively against GI parasites. Grazing of animals in rangelands containing trace elements rich forages increase the resilience against parasitic infections particularly in resource poor countries like Pakistan. Keywords: Gastro-intestinal parasites, Trace elements, Sheep, Prevalence, Sialkot, Pakistan

Biography

Hafiz Muhammad Rizwan, a doctoral student in the Department of Parasitology at the University of Agriculture, Faisalabad, came to UC Davis to help improve those diagnostic techniques. His USPCAS-AFS exchange research was housed in the lab of Dr. Jeroen Saeij, an associate professor and toxoplasmosis researcher at the UC Davis School of Veterinary Medicine. Mr. Rizwans research goal is to devise an improved serological assay that would use polymorphic peptides from Toxoplasma proteins for assessing the virulence of the sample strain. His initial work at UC Davis yielded intriguing results, but found that his peptide serum needed additional calibration to recognize specific strains of toxoplasma. Mr. Rizwan also worked on research related to the CRISPR/Cas9 gene editing method he targeted and removed a specific gene in his toxoplasma gondii samples. After developing guide RNA strands, preparing plasmids, and then cloning them, he was able to use a plaque assay to quantify the toxoplasma viability with the new gene sequence.These diagnostic techniques will help us in Pakistan, says Mr. Rizwan. Hopefully, the plan is to develop the peptides and develop a dipstick method: just place samples into the serum, and that will alert you to which strain of Toxoplasmosis is present. Along with his scientific investigation, Mr. Rizwan picked up several soft skills during his USPCAS-AFS exchange. He has been invited to present his results to conferences in Malaysia and Turkey this fall. Mr. Rizwan also drafted and submitted several research papers based on his research experiences while at UC Davis, and learned about how to conduct lab group meetings, arrange journal clubs, review ongoing research articles, and track his own research through meticulous lab notebooks. He plans to train his colleagues at UAF on several of the same techniques.

Speaker
Hafiz Muhammad Rizwan / University of Agriculture, Faisalabad, Pakistan

Abstract

Infectious bronchitis virus (IBV) causes respiratory disease in poultry as well as affecting avian renal and reproductive systems. Controlling of IBV is mainly based on vaccination program. Current available lived attenuated or killed vaccines have been challenged by their effectiveness due to IBV variants and lack of cross-protection. In our studies, to address this issue, we designed novel IBV vaccines by using a highly innovative platform called self-assembled peptide nanoparticle (SAPN). Spike protein comprises major antigenic determinants that induce neutralizing antibodies which makes it a major target of vaccine design. We engineered the coiled-coil sequence of IBV onto the trimeric coiled-coil of several versions SAPNs. This design presents spike protein of IBV epitope in a specific manner to induce immune response against IBV infections. Four week-old SPF chickens were given 100 μg IBV-SAPN vaccine or refolding buffer intramuscularly. Each animal received two boosters two weeks apart. Chickens were bled and sera were separated to assess the immunogenicity. Immunogenicity study is being assessed with assays including ELISA, virus neutralization, lymphocyte proliferation, flow cytometry. SPF Chickens vaccinated with the IBV-SAPN induce higher levels of antibodies than unvaccinated control groups. Vaccine efficacy in chickens will be evaluated by challenging with different virulent strains of IBV in future.

Biography

azhar I Khan has did his Postdoctoral Fellowship in Molecular Biology, University Of Georgia, Athens in the year 1987-1988 and Ph. D. in Comparative Pathology, University of California, Davis, 1987. Mazhar I Khan currently works at the Department of Pathobiology and Institute of Material Science, University of Connecticut. Mazhar does research in Bioinformatics, Biostatistics and Biotechnology. Their current project is Immunogenicity Studies on Nanoparticle Based Vaccines for Bird Flu.He has awarded with many awards such asChinese Government Friendship Prestigious Award, Beijing, China, 2013,Qilu Friendship Award, Shandong Province, China, 2013,Pakistan Research Presentation Award, Islamabad, Pakistan, 2008,Guangxi Provincial Scientific Friendship Award, 2006,Listed in Whos Who in the South East, 1990-1994,Listed in Whos Who of Emerging Leaders in America, 1993

Speaker
Mazhar I. Khan / university of Connecticut, USA

Abstract

MThe first major objective of this study is to explore the service quality level of Public Hospitals in the city of KARACHI from perspective of patients. Secondly, the satisfactions level of the ailing patients towards the services provided by these health care government sectors in general is investigated. The measurement instrument used in this study is based on Questionnaires. Customer satisfaction and service quality are often treated together as functions of customers perceptions and expectations. Research has shown that high service quality contributes significantly to customer satisfaction and customer delight. This study empirically explores the relationship between hospital quality management and service quality performance for a sample of patients of government health care hospitals in the city of Karachi. SERVQUAL model has been adopted to encompass various aspects of service quality. The study has been undertaken to demonstrate the Gaps for measuring patients perceptions-expectation of health care services quality in GOVT hospitals in the city of Karachi. . In this study an attempt has been made to explore the service quality gap which is called Gap Score by means of making a comparison between customers expectations and their actual perceptions towards the services and the government hospital patients are treated in. The purpose of this research is to provide review of the SERVQUAL research in measurement of health care service quality, to obtain information about quality parameters of services provided by GOVT hospitals of Karachi & to find out as to how much these parameters rate are as per the expectations of the patients. A sample size of 150 ailing patients is taken from department to department in different government hospitals situated in the city of Karachi. The results have shown an alarming situation where government hospitals are far away from the patients expectations. Furthermore there is an extreme need for training in the area of public hospital staff and their attitudes towards the ailing. Looking at the situation it is highly recommended that the government should take a country wide initiative to launch special programs where hospitals internal structures and work process are re-organized on the principles of quality management practices, through the introduction of ISO Programs. This would help in improving the service quality at each stage of the services provided by these hospitals. .

Biography

Dr. Hira Sabir Malik has her expertise in evaluation and passion in improving the health and wellbeing. Her open and contextual evaluation model based on responsive constructivists creates new pathways for improving healthcare. She built this model after years of experience in research, evaluation, teaching and administration both in hospital and education institutions. The foundation is based on years of evaluating services of the health care organization, which is a methodology that utilizes the previous generations of evaluation: measurement, description and judgment. It allows for value-pluralism. This approach is responsive to all stakeholders and has a different way of focusing.

Speaker
Hira Sabir Malik / BAHRIA UNIVERSITY KARACHI CAMPUS, PAKISTAN

Abstract

Background: Mixed infections of enteric viruses and parasite can infected the human alimentary canal and quite complex physiological changes can result from such infection. Objectives: To study the association between enteric viruses and giardiasis among diarrheal children as well as to clearly the relationship with Giardia lamblia genotypes in Diyala province, Iraq. Patients and methods: Descriptive cross sectional study which was conducted in Al-Batool Teaching Hospital for Maternity and Children in Baqubah city, during the period from June 2017 till November 2017. One hundred and sixty stool samples were collected from children with gastroenteritis by use clean and dry container; then samples were screened for rotavirus, adenovirus, astrovirus and norovirus using Cer Test Biotec, and Giardia lamblia by ELISA kits; the positive Giardia lamblia samples were further analysed by nested polymerase chain reaction. Results: Laboratory analysis showed rotavirus was detected in 20%, adenovirus in 18.75% and norovirus in 13.75% while no positive result with astrovirus. The Giardia lamblia were detected in 42 cases (26.25%), amplification triose phosphate isomerase (tpi) gene was successful in 18/42 (66.66%) samples, 8 (28.57%) contained genotype A and 20 (71.43%) samples contained genotype B. Majority of infected children was less than five years and males. Conclusion: Rotavirus infection rate amongst children in Diyala appears to be relatively high than other enteric viruses. Co-infection with Giardia lamblia type B have important role.

Biography

Speaker
Areej Atiyah Hussein / University of Diyala, Iraq

Abstract

Statement of the problem : New cases of polio, Measles and rubella. Began to appear in the Mediterranean region, including Jordan due to breakdown of primary health care system and a lot of children in Syria is definitely infected with these major diseases caused by the civil war. World Health Organization (WHO) adopted a vaccination campaigns in neighboring countries closed to Syria since 2 November 2015 to 21 November 2015in order to control the spread of the disease through the reservoir (refugees) getaway from Syria to neighboring countries such as Jordan. Objective: Control , prevention and eradication of polio, measles and rubella. Method: a vaccination campaign for these major diseases (polio, measles and rubella) in the period between 2 November to 21 November 2015carried out this campaign through the Ministry of Health Jordan (MOH) , Royal Medical Services (RMS), United Nations Relief and Works Agency (UNRWA) and non-governmental organizations (NGOs) health centers across the Jordan. Target population: The campaign targets the vaccination children against polio for the age group of one day to five years, while the targeted immunization with vaccine of measles and rubella for the age group of 6 months to 20 years for all Jordanians and non- Jordanians residing on the land of the Kingdom regardless of taking them to these vaccines earlier in the campaign. Result : total number of children and young people within the age group of the day and until the age of 20 years had vaccination (2,500000), among of those (56500) vaccinate by RMS as well as about ( 46000 ) measles and rubella , (10500) polio vaccine. Conclusion: high vaccination coverage as well as a huge number of people from under the age 20 years they attended Ministry of Health, Royal Medical Services, UNRWA, and Non-governmental Organizations health care center in Jordan to get vaccination.

Biography

Speaker
Zeyad Bataineh / King Hussein Medical Center, Jordan

Abstract

Pharmaceutical Microbiology: Essentials for Quality Assurance and Quality Control presents that latest information on protecting pharmaceutical and healthcare products from spoilage by microorganisms$ and protecting patients and consumers! +ith both sterile and non'sterile products$ the effects can range from discoloration to the potential for fatality! "he book pro#ides an o#er#iew of the function of the pharmaceutical microbiologist and what they need to know$ from regulatory filing and %MP$ to laboratory desig management$ and compendia tests and risk assessment tools and techni&ues! "h aspects are discussed through a series of dedicated chapters$ with topics co#ering auditing$ #alidation$ data analysis$ bioburden$ to)ins$ microbial identification$ culture media$ and contamination control!

Biography

Speaker
Abdallah /

Sessions:

Parasitology

Abstract

"Formation of knowledge of causes of infectious diseases and development of specific prevetion against them in Hungary from the beginning till 1900 Ralovich Bela Ministry of Welfare (retired), Budapest, Hungary Abstract During our history the following terms were used as the cause of an infectious illness besides the will of God: the first was Egyeduti Gergelys death of sky and latter Frankovith Gergely s sticker, poison and pestiferous substance. Since their opinions the next names can be read in publications: animacula, chaotica, fungus, hostes, infusoria, insect, living plant, noxia, poison, plague, sickly product, sticker contagium, seed, unknown daceyed organic substance, vermiculus, virus and so on. These terms were applied not only before 1850 when the role of B. anthracis was verified but after it also for years."

Biography

Ralovich Bela was born in 1937 at Pecs (Hungary). He received his M.D. diploma in the Medical University of Pecs. He started to work for the Institute of Microbiology (1961-1975) near professor Rauss and latter for the Institute of the Public Health and Epidemiology (1975-1988). He thought medical students and his main research fields were: enteric bacteria, salmonellosis, salmonella immunity, listeriosis, food microbiology and microbial flora of surface waters. He performed researches in the U.K. as well as in Russia and presented scientific lectures in 14 countries. He was a WHO expert for listeriosis and organized two International Listeriosis Symposia. He received Ph.D. (salmonellosis) in 1973 and D.M.S. (listeriosis) in 1986 from the Hungarian Academy of Sciences (HAS). In 1988 he became an expert in the Research Institute of the Hungarian Meat Indistry (1988-1992) and finally a senior executive of public health in the Ministry of Welfare (1992-1999) at Budapest. In 1999 he retired. He published 4 books, more lecture notes, some chapters and more than 165 articles. He was the member of several Hungarian and International (American, British, Taxonomical, Food Microbiological and so on) Societies and is an active member of the Hungarian Society for Microbiology still. His scientific activity was recognised with different awards (Markusovszky Diploma, Outstanding Scholar Award of the HAS (three times), CDC Medal (USA), Manninger Medal and the Golden Cross of the Hungarian Republic).

Speaker
Ralovich Bela / Ministry of Welfare (retired), , Hungary

Abstract

Enteric viruses and parasites are entities of infectious nature that display tropism towards cells of the gastrointestinal and epithelial tract, being associated with the production of gastroenteritis, hepatitis, conjunctivitis among other pathologies. Due to their composition they are more resistant than the bacteria to conventional disinfection water process and therefore they are transmitted by this hydric resource. This generates the need to do a constant monitoring that allows monitoring water quality from the virology and parasitology point of view. Through this study, a diagnosis of the presence of Hepatitis A Virus (HAV) Rotavirus (RT) and Giardia lamblia was performed before and after the treatment process in four treatment plants located at the department of North of Santander, Colombia. In addition, we analyzed the quality of the water sources from which the plants are supplied. For this purpose, immunomagnetic separation technique was used to concentrate and separate the pathogens from raw and drinking water samples. To confirm the presence of viruses reverse transcriptase-polimerase chain reaction (RT-PCR) was used to identify the presence of the two enteric viruses whose genome is based on RNA. The results allowed determining the presence of RT, HAV and Giardia lamblia in all the raw water samples, whereas only HAV was present in one of the drinking water treatment plants. In this way, it was possible to conclude that fluoro-imnunomagnetic technique was efficient in capture, concentration and even determination of tested pathogens in raw water and drinking water. Keywords Enteric diseases, viruses, parasites, raw water, drinking water, immunomagnetic technique.

Biography

Microbiologist from the University de Pamplona, N de S. Colombia. She got a master degree in Molecular Biology and Biotechnology from the same university and a PhD in Nanoscience and Nanotechnology from the Universitat Rovira i Virgili, Tarragona, Spain. She had done internships at the Universites of Stuttgart, Germany and Technical University of Denmark, Denmark related to Nanotechnology field. She is currently assistant professor at the Department of Microbiology of the Universidad de Pamplona and the leader of the research group in Nanotechnology and Sustainable Development. She is also member of the National Network in Nanotechnology of her country and her research interest is focused towards the development of biosensors with analytical applications. She had also interested on the use of silver and magnetic nanoparticles with different biological applications supported by several publication in this area.

Speaker
RAQUEL AMANDA VILLAMIZAR GALLARDO / University of Pamplona , Colombia

Abstract

In this article, we developed a hybrid model by coupling a mathematical model(EBM) of the intra-host phase of Rift valley fever(RVF) and an agent-based model(ABM) of the interactions between animals, mosquitoes, pond water and climate. The mathematical model describes the dynamics of healthy cells, infected cells, virus populations and immune effector cells. The aim of this work is to create a virtual environment that EBM and ABM models allow us to follow the evolution of the RVF after animal infection. The other objective of this work is to minimize the storage capacity of the data used by all agents in the computers central memory. We present a mathematical analysis of the model. We calculate the basic reproduction number R0. We compute the equilibrium points and their stability. We present the results of the numerical simulations to illustrate and validate the theoretical results. The developed model can serve to evaluate the rate of infected cells and viruses in the organism of the animal at each step of the evolution of the disease.

Biography

Speaker
Python Ndekou Tandong Paul / Anta Diop University, Senegal

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