Nanotoxicology is a science that has its demand increased as much as the necessity for new safety assignments of nanotechnology needs to human beings and its effects in the environment. Nowadays new nanotechnology products have been developed by the industry and scientific researches supplying either industrial and medical necessities, but this new products have different compositions, electrical charges, sizes, shapes, and other characteristics that are determinant to biological effects. For example, the interaction between nanotechnologies and biological systems can promotes toxic effects, blocking channels or enzymatic in active site, producing pro-oxidants, and activating immune response. For those reasons, international agencies and governments are investing resources to create effective protocols that will be able to insure the safety of nanotechnology products. The heart is an unique organ, having different type of muscles cells, a very specific electrical activity, and also self-control own its beats. Likewise, heart toxicity has different requirements for safe assignments, and it is still unknown if the usual tests applied nowadays are sufficient to ensure the safety of nanotechnology use. Therefore, it is important to understand the risks of the nanotechnologies to the heart