A new systems approach to diseased states and wellness result in a new branch in the healthcare services, namely, personalized and precision medicine (PPM). To achieve the implementation of PPM concept, it is necessary to create a fundamentally new strategy based upon the subclinical recognition of biomarkers of hidden abnormalities long before the disease clinically manifests itself. Each decision-maker values the impact of their decision to use PPM on their own budget and well-being, which may not necessarily be optimal for society as a whole. It would be extremely useful to integrate data harvesting from different databanks for applications such as prediction and personalization of further treatment to thus provide more tailored measures for the patients resulting in improved patient outcomes, reduced adverse events, and more cost effective use of the latest health care resources including diagnostic (companion ones), preventive and therapeutic (targeted molecular and cellular) etc. A lack of medical guidelines has been identified by responders as the predominant barrier for adoption, indicating a need for the development of best practices and guidelines to support the implementation of PPM! Implementation of PPM requires a lot before the current model “physician-patient” could be gradually displaced by a new model “medical advisor-healthy person-at-risk”. This is the reason for developing global scientific, clinical, social, and educational projects in the area of PPM to elicit the content of the new branch.
Sergey Suchkov graduated from Astrakhan State Medical University and awarded with MD, then in 1985 maintained his PhD at the I.M. Sechenov Moscow Medical Academy and in 2001, maintained his Doctorship Degree at the Nat Inst of Immunology, Russia. From 1987 through 1989, he was a senior Researcher, Kolt-zov Inst of Developmental Biology. From 1989 through 1995, he was a Head of the Lab of Clinical Immunology, Helmholtz Eye Research Institute in Moscow. From 1995 through 2004, a Chair of the Dept for Clinical Immunology, Moscow Clinical Research Institute (MONIKI.DrSuchkov has been trained at: NIH; Wills Eye Hospital, PA, USA; Univ of Florida in Gainesville; UCSF, S-F, CA, USA; Johns Hopkins University, Baltimore, MD, USA. He was an Exe Secretary-in-Chief of the Editorial Board, Biomedical Science, an international journal published jointly by the USSR Academy of Sciences and the Royal Society of Chemistry, UK. At present, Dr Sergey Suchkov is a Chair, Dept for Personalized and Translational Medicine, I.M.Sechenov First Moscow State Medical University. He is a member of the: New York Academy of Sciences, USA; American Chemical Society (ACS), USA; American Heart Association (AHA), USA; EPMA (European Association for Pre-dictive, Preventive and Personalized Medicine), Brussels, EU; ARVO (American Association for Research in Vision and Ophthalmology); ISER (International Society for Eye Research); PMC (Personalized Medicine Coalition), Washington, USA.
Dr Gamlen has had an extensive career spanning over 40 years in scientific research and tablet development in a range of contexts including Development, Clinical Trials Manufacturing and Production. Formally the Head of Tablet Development at the Wellcome Foundation Ltd for 15 years, and a consultant to Vanguard Medical Ltd, Michael recognised and identified the need to improve tablet quality under controlled laboratory conditions. An excellent communicator, Michael has an international reputation as a presenter and speaker on tableting, process development and related technical areas. An honorary lecturer at De Montford University School of Pharmacy, he also acts as a Pharmaceutical Expert Witness in a broad range of pharmaceutical cases relating to drug substance properties, tablet formulation and development, and controlled release product development.
Nitric oxide (NO) is a gaseous messenger with a short half-life (~ 1 s), which plays a pivotal role in vascular tone homeostasis. It is continuously produced in the endothelium of vessels from arginine through catalytic action of NO synthase. Diet is also a source of NO species, mainly as nitrate and nitrite ions. During ageing and in the case of cardiovascular diseases (CVD) related to endothelial dysfunction, endogenous NO production decreases.Administration ofNO donor, particularly S-nitrosothiols (RSNO) which mimic physiological reservoir of NO, appears promising approach to restore the NO pool in preventionof CVD but constitute a real challenge. Drug delivery systems are currently developed to control the dose, sustain the RSNO release and finally improve efficiency. An overview of this topic is proposed.
Dr. Anne Sapin-Minetis an Associate Professor in Pharmaceutical Technologiesin Nancy, France (research unit EA 3452 CITHEFOR). She has over 12 years experience in teaching conventional and innovative drug delivery systems in University of Lorraine. She is author of more than 20 papers published mainly on international journals.Currently, Dr. Anne Sapin-Minet is engaged in working on the development of new therapeutic strategies for Nitric Oxide donor delivery (mainly in the context of vascular pathologies). Control of the release of NO is optimized, depending on the route of administration, using particulate formulations (nano or micro).
Moringa oleifera is a tropical and subtropical deciduous tree native of South Asia. The leaves are rich of many bioactive compounds as polyphenols, vitamins and literature data showed many pharmacological activities as antibacterial and anti-inflammatory. The aim of this work was to develop a formulation for wounds treatment using Moringa oleifera extract as active ingredient. This was prepared by an eco-friendly method using a hydroalcholic solution as solvent. The extract showed goog antioxidant and antibacterial activities. S. epidermidis, S. pyogenes, S. aureus and S. faecalis, strains commonly responsible for wounds infection, were the most sensitive. These results suggested that the extract could be useful for wounds treatment. A confirmation of this was obtained from the healing activity assay (scratch test) performed on human keratinocytes cell line. It was observed that a water solution of the extract (0.4 mg/ml) is able to stimulate the cells growth and to promote the wound closure within 24 h (faster compared to the control, untreated cells). Thus, the extract was formulated as swellable polymeric microparticles to be applied on exudating wound and to form a film closing the wound until complete healing. The microparticles were prepared by spray drying using a water solution of the extract, chitosan (as bioadhesive polymer) and Soluplus®. The latter allowed to obtain wrinkled microparticles, useful for an intimate contact with the tissue. A preliminary evaluation of microparticles performances showed their rapid swelling after contact with wound forming a protective gel layer. Further studies must be performed to evaluate the microparticles performances.
she is a regular teacher of): Pharmaceutical Technology, Socioeconomy and Law for Industrial Pharmacy degree (II level), University of Perugia (Dept. of Pharmaceutical Sciences) Previous teaching: Pharmaceutical industrial processes and equipments, Pharmaceutical Technology for Industrial Pharmacy course (II level) and Quality Control course (Ilevel). Cosmetic Products for Enviromental and Occupational Prevention Techniques course (I level) Biotech Production for Biotechnology course (I level) She is director and didactic coordinator for II level master (post graduation) in Pharmaceutical Technology and Regulatory Affairs. She is a member of the teaching board of international and industrial doctorate (Ph. D. Course) in Pharmaceutical Sciences, university of Perugia (Italy). She graduated in Pharmacy in 1986 and obtained her Ph.D. graduation in 1992. She is author of many research papers (more than 150) published mainly on international journals. She was a member of Bioethic Committee of the University of Perugia and Arezzo Hospital. She founded an university spin off in 2008 for production and studies of nanomaterials for pharmaceutical and cosmetic products.
The occurrence of wound is almost inevitable making it a global clinical challenge; however, wound care and management may be dealt with in an advanced and natural manner, reducing escalating healthcare expenses. Our experimental work involves developing a spongy scaffold composed of locust bean gum and gelatin cross linked with glutaraldehyde, containing resveratrol as model drug. The use of natural biodegradable polymers and polyphenolic drug will ensure minimum toxicity to the wound site. The physicochemical, in-vitro and in-vivo properties of the scaffold were evaluated. Substantial swelling, fluid retention and absorption were exhibited, needed to keep the wound area moist for rapid wound healing. Highly porous nature of the spongy scaffold absorbs large volume of wound exudates and increases the distribution of nutrients and medium for the cells that get attached on it. The scaffold showed good antimicrobial activity against both gram-positive and gram-negative bacteria as well as impermeability towards microbes, parameters essential in preventing secondary infection which is largely responsible for delayed wound healing. When applied to the bare wound site, the scaffold serves as a mechanical support and allows cells to get attached underneath and start remodeling the wounded area, augmenting healing process. Resveratrol entrapped inside the scaffold matrix was released over a prolonged period of time without any initial burst, thus ensuring good antioxidant activity. SEM images of the transverse section of scaffold revealed cross linked internal environment, confirming its porous nature. Thus, the prepared spongy scaffold can be established as an excellent aid in wound healing.
Dr. Amalesh Samanta has completed his Ph.D in Pharmaceutical Microbiology from Jadavpur University. He is working as a Professor in the Dept. of Pharmaceutical Technology of Jadavpur University, Kolkata, India. He has published 86 papers in reputed journals and has been serving as Coordinator of M.Pharm course in Clinical Pharmacy and Pharmacy Practice of Jadavpur University. His research interest involves formulation development and dosage designing. He is associated with Indian Pharmaceutical Association, American Chemical Society (ACS).
Objectives; the abuse of drugs can have serious ramifications on a person’s physical health, mental health, and overall well-being. Aim of the work is to determine the prevalence of some drugs among patients from the laboratory point of view. Methods; the sample size for this study was five hundred patients with acute poisoning by some drugs of abuse. In this study all patients were subjected for detection of drugs of abuse in urine by EMIT system and Gas Chromatography / Mass Spectrometry (GC/MS) for confirmation of the obtained results. Results; the study revealed that the percentages of positive urine samples by Enzyme Multiplying Immunoassay Technique (EMIT) confirmed by Gas Chromatography / Mass Spectrometry (GC/MS). Conclusion; presence of these drugs has a serious effect on man health, consequently his environment. Recommendations; it is recommended that public health intervention aimed at preventing drug use among inhabitants should be designed to raise awareness about the negative effects of drug abuse.
Summary. Ketamine anesthesia can be the reason of damage of the central nervous system (CNS) in the postoperative period. It could lead to the development of postoperative cognitive dysfunction, which can appear in patients of different age groups and in cases with a non-aggravated psychoneurological history. The purpose of this study was to evaluate the effect of the intranasal drug form of the IL-1b antagonist (ARIL) (1% homogeneous transparent solution with low viscosity) on the formation of cognitive deficits in rats after ketamine anesthesia. Materials and methods. Experiment was carried out on 50 6-monthes old Wistar rats of both sexes weighing 220-290 g. Ketamine anesthesia was modeled by administering 100 mg / kg of ketamine intraperitoneally. After the animals got out of anesthesia, they reveived ARIL (7.5 mg / kg) once intranasally, intact and control group of animals received physiological saline in a similar volume intranasally. On the second day after anesthesia, the motor and search activity was assessed using the “Open Field” technique and began to train the animals in the labyrinth for 10 days. We evaluated the reference memory, as well as the working memory and the number of operating memory errors with the help of the radial labyrinth LE760 (AgnTho's, Sweden). The capture and recording of the image was carried out using a color video camera SSC-DC378P (Sony, Japan). The video file was analyzed using Smartv 3.0 software (HarvardApparatus, USA). Results. Ketamine anesthesia led to an increasing of the anxious behavior of animals, excitability (increase in the duration of inactivity and the number of fading cases, increase in the free distance, the number of columns at the wall, decrease in grooming and defecation), and to the deterioration of research and search activity on day 2, as well as in more remote time (10 days). It should be noted that mnestic functions of animals were decreased. We noticed an increase in the number of errors in the reference and working memory tests. Intranasal administration of ARIL to animals after ketamine anesthesia led to reducing of indicators of anxious behavior and excitability of animals (decrease in the number of fades and inactivity, free distance, columns at the wall), to increasing research activity. Therapy also showed a pronounced antiamnestic effect (reduced errors of reference and working memory) and increased the ability of animals to learn (decrease in high and increase low activity). Conclusions. Obtained results are experimental grounds for further study of the effect of the intranasal form of IL-1b antagonist ARIL for correction of cognitive-mnestic dysfunction after ketamine anesthesia.
Dr. Michael J. Gonzalez is Professor at the Nutrition Program, School of Public Health in the Medical Sciences Campus, University of Puerto Rico and Adjunct faculty at the University of Western States And EDP University. Dr. Gonzalez is a Fellow of the American College of Nutrition, and has authored over 200 scientific publications. He serves as a member on multiple scientific Editorial Boards. He has served as consultant for several companies where he has been responsible for designing formulations of nutritional supplements and pharmaceutical products. He has also been a consultant for The Center for the Improvement of Human Functioning (now Riordan Clinic), in Wichita, Kansas. He has obtained several research awards for his work on Nutrition and Cancer. He is currently Co- Director of RECNAC II project, and Research Director of the InBioMed Project Initiative. Dr. Gonzalez also serves as a nutrition consultant to the Puerto Rican Basketball National Team and is part of the Medical Commission of the Puerto Rican Basketball Federation. He was part of the Medical Staff of the Vaqueros de Bayamon professional basketball team in Purto Rico. He is in a part time clinical practice with Dr. Miguel J Berdiel in Ponce, PR. In December 2013,Dr. Gonzalez was exalted as Distinguished Ponceño in Medicine. In 2015 he was selected as member to the prestigious Puerto Rican Academy of Arts and Sciences and to the Iberoamerican Academy of Culture and Sciences. Doctor González is one of the founders of InBioMed, leaders in the development of non-toxic chemotherapy treatments for cancer. The findings of their work with Intravenous Vitamin C as an anti-cancer agent, published in 2002, were confirmed by the NIH in 2005. They published the first Phase-I clinical study utilizing Intravenous Vitamin C for treatment of terminal cancer patients in 2005, and also published in 2005 the most comprehensive review on Vitamin C and Cancer, as a follow-up on the work of two times Nobel Laureate, Dr. Linus C. Pauling. They have brought many new concepts into the scientific field, such as the Bioenergetic theory of carcinogenesis, the systemic saturation phenomenon of intravenous vitamin C, the metabolic correction concept for disease treatment and prevention. Dr. Gonzalez was inducted to the International Hall of fame of Orthomolecular Medicine April, 2016. He is one of the first hispanoamerican and Puerto Rican to be inducted to the International Hall of fame of Orthomolecular Medicine. Books: “I Have Cancer What Should I do: The Orthomolecular Guide to Cancer Management” provides evidence based recommendations for an integrative approach for those dealing with cancer and seeking to improve quality of life and survival. In 2014 ” New Insights Vitamin C and Cancer” a book on the scientific evidence on Vitamin C as an anticancer agent for Springer-Larger. Dr. Gonzalez is actually finishing a book on Metabolic Correction and Diabetes and writing a chapter on metabolic correction and disease for a Functional medicine textbook.
Advances in biotechnology and genetic research have led to increased emergence of peptides and proteins as a major class of therapeutic agents. Therapeutic proteins are effective at low dose and gaining increased interest as a drug molecule eg. insulin, thyroid hormone and clotting factor. The ability to deliver these compounds systemically using convenient and effective delivery system has been a matter of concern. The biophysical, biochemical and physiological characteristics of proteins and peptides like size, biological half life, immunogenicity, conformational stability, dose requirement site and rate of administration, pharmacokinetics and pharmacodynamics is very challenging and a tough task. Protein and peptides are charged molecules cannot penetrate lipophilic plasma membrane (transcellularly), paracellular transport through the tight junction is also fairly limited due to large molecular size ï¿½500 Da and the most common parenteral route is associated with pain as well. A novel carriers as a potential system for delivery of protein and peptide drug is Aquasomes. They are self-assembled solid drug carriers consist of ceramic core whose surface is non-covalently modified with carbohydrates to obtain a sugar ball which is further exposed to adsorption of therapeutic agent. The core provides structure stability to largely immutable solid. Disaccharides tend to stabilize biological macromolecules due to quasi-aqueous environment which prevent dehydration induced denaturation. Thus aquasomes offer an attractive mode of delivery of proteins and peptides providing conformational stabilization overcome physical, chemical instability and poor bioavailability and lessen the potent side effects to adsorbed drugs.
Working Papers: With Seetharaman.r Application of Chemometrics and Experimental design for the estimation of antihypertensives, antidiabetics and their combinations With Kavitha .J ï¿½Application of Multivariate Calibration for the estimation of harmful ingredients present in Food, Cosmetics and Pharmaceuticals causing infertility Work in Progress: Mr.R. Seetharaman : Application of Chemometrics and Experimental design for the estimation of cardiovascular and Antidiabetic Drugs. Mr. K. Manikandan : Chemometric applications for Estimation of novel drugs and its combinations. Mrs. J. Kavitha : Application of chemo metrics and experimental design for the estimation of Harmful agent causing infertility in foods and cosmetics and pharmaceuticals. Mrs. J. Jayasutha : Evaluation of efficacy, safety and cost effectiveness of various combination therapy in hyperinsulinemic polycystic ovary syndome. Mrs. K.S. Koilambiga : Design, Development and Validation of HPLC and HPTLC method for the novel drug combinations. Mr. V.J. Vishu Varathan : A Comparative study on the therapeutic effect of formononetin and formononetin loaded solid lipid nanoparticles in the treatment of lung cancer Mrs.M.Rama : Attenuation of PCOS ï¿½ Polycycstic ovarian syndrome using Herbal Formulation in rat model Academic Experience: Taught UG, PG grades in Pharmacy for 22 years. Currently as Dean cum Head Of Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology (formerly known as SRM University)- India. website www.srmuniv.ac.in. Other Professional Experience: As a Dean of SRM Pharmacy College, SRM Institute of Science and Technology (formerly known as SRM University) India, for the past 20 years.1996-Till date Served as a Principal with Velï¿½s College of Pharmacy, The Tamil Nadu Dr. MGR Medical University, Chennai, India for four years.1992-1996 Lecturer at CLBM College of Pharmacy Chennai Tamil Nadu India 1990-1992. First Assignment as Lecturer at JSS College of Pharmacy Ooty Tamil Nadu India 1988-1990. Key responsibilities included program development and annual program funding allocation. Doctorial Committee - 3 Universities PCI Inspector Achievements and Awards: Inspector for the Pharmacy Council of India, New Delhi ï¿½ India. Inspector for the Tamil Nadu DR.M.G.R Medical University , Chennai ï¿½ India.
Pharmaceutical, Biotech and Medical Device companies are constantly evaluating how decisions at the US ,Europe, Turkey and emerging markets are taken at the federal and state level and how it will impact provider and patient access and coverage to their therapies. To gain these critical insights, Government Affairs & Medical affairs teams require speaking with those on the frontlines of healthcare delivery - physicians, allied healthcare professionals, payers, and patients. New generation government engagement and commercial diplomacy approach developed by ICG ( Istanbul Consulting Group) will be provided with unique cases. Dr. Yavuz Selim SILAY a global leader and seasoned expert in Government engagement, Commercial Diplomacy, Corporate Communications, Government affairs & Medical Affairs will discuss how companies utilizing Government Affairs and Medical affairs teams should engage decision makers, payers,physicians and acquire these insights in a convenient, cost-effective, and compliant manner in this current changing global regulatory landscape.
Linum usitatissimum is a cold temperature herb (Linaceae family) which seeds are rich of unsaturated fatty acids (oleic acid, linoleic acid, linolenic acid), flavonoids, lignans, etc. These molecules show many activities as antioxidant, anti-inflammatory, antibacterial and healing suggesting the potential application of flaxseeds in health field. The aim of this work was to develop and characterize a suitable formulation based on Linum usitatissimum extract for skin ulcers treatment. Two different extracts were prepared, a dry extract (DE) obtained at 60°C using EtOH 60% as solvent and an oil extract (OE) obtained at room temperature using EtOH 96% as solvent. From the antibacterial activity assay DE and OE resulted particularly active agains S. pyogenes and DE also against S. aureus. The in vitro healing activity assay performed on human keratinocytes showed that DE (water solution 0.15 mg/ml) is able to stimulate cells growth. Thus, DE and OE were co-formulated in a novel emulgel in which DE was solubilized in the external water phase (W) and OE incorporated in the internal oil phase (O). W was viscosized by a water solution (1% w/v) of FG 90 chitosan (chitosan 99.97% deacetylated). This polymer alone showed interesting antibacterial activity against S. pyogenes, P. aeruginosa and E. coli. The antibacterial assay performed on the emulgel showed a synergistic effect of the employed materials demonstrated by the increased inhibition halo measured. Ongoing studies are focusing on the deeper characterization of the emulgel.
In the last decades, there was a re-emergence of the interest for natural products, in particular bioactive herbal compounds, with medicinal interest for topical formulations. The aim of the present study was to provide scientific evidence supporting the medicinal use of Sambucus nigra extracts and to assess whether nanotechnology may improve their activities. Flowers and fruits of S. nigra were collected at the same area of northern Portugal. Twenty-seven extracts of fresh flowers, as well as fresh and dried berries obtained using different solvents and methodologies. Simultaneously, different types of nanocarriers (polymeric, metallic and vesicles) were prepared in order to further encapsulate the most promising extracts. The extracts were screened for antioxidant, enzymatic inhibition and anti-inflammatory (in vitro and in vivo) activities. Results suggest the biological activities of S. nigra extracts are highly dependent on the plant organ (flowers, dry berries or fresh berries) and the extraction method (ultrasounds, microwaves or supercritical fluid extraction). The most promising extracts were those resulting from the methanol (E1) and ethanol (E4) extraction of flowers using ultrasounds. E1 showed higher antioxidant activity (93 ± 3%). Both extracts showed collagenase inhibition (94 ± 1% for E1 and 88 ± 3% for E4) and in vitro anti-inflammatory activities (97 ± 7% for E1 and 102 ± 4% for E4). E1 and E4 extracts were also assayed in vivo for anti-inflammatory activity. E1 showed the best anti-inflammatory activity with 37 ± 9% and 108 ± 63% after oral and topical administration, respectively. E4 displayed an in vivo anti-inflammatory activity by oral and topical administration of 27 ± 5% and 74 ± 34%, respectively. Neither E1 or E4 were cytotoxic, when tested on HaCaT and THP-1 cells. HPLC-ESI-MS/MS was performed in order to identify the major compounds present in those extracts that have revealed the highest polyphenol content. Several flavonoids were identified, namely rutin (major compound), isoquercetin, isorhamnetin-3-O-glucoside and narigenin, among others. Some nanocarriers have been studied as potential carriers for the S. nigra extracts. Thus, polymeric nanoparticles (NPs) based of poly (D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL) NPs, and ethosomes were successfully prepared. PLGA NPs were obtained by the emulsification/solvent diffusion method; PCL NPs were prepared using the solvent-displacement method; and ethosomes were prepared by the cold method. All the carriers showed monodisperse populations (PI less than 0.3) and displayed a spherical morphology accessed by scanning and transmission electron microscopy as well as, by atomic force microscopy. Finally, E1 extract was encapsulated into above-mentioned nanocarriers and the encapsulation efficiency was determined using rutin as a marker, revealing that PLGA and PCL nanoparticles and ethosomes encapsulated 47 ± 20%; 96 ± 5% and 72 ± 31% of the extract, respectively. The antioxidant activity and polyphenol content was again assessed in free E1 form and loaded in different nanocarriers. Results are shown in Fig. 1. Figure 1 – Antioxidant activity of the extract, empty and E1-loaded nanocarriers (%, mean ± SD; n=3), Ascorbic Acid was used as positive control. In a near future, the biological activity (collagenase inhibition, anti-inflammatory and toxicity assessment) of E1-loaded nanocarriers will be assessed using the methods previously used for extracts evaluation. After full characterization, E1-loaded nanocarriers will be incorporated into vehicles appropriate for skin application. Acknowledgements: Régiefrutas for the supply of S. nigra flowers as well as, the financial support; FCT and Portugal 2020 - Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa - RNEM; LISBOA-01-0145-FEDER-402-022125).
The evaluation of pharmaceutical raw materials and finished products for impurities and degradation products is an essential part of the drug development and manufacturing testing process. Additionally, toxicological information must be obtained on any drug-related impurity that is present at a concentration of greater than 0.1% of that of the active pharmaceutical ingredient (API). In pharmaceutical QC and manufacturing, impurity analysis has traditionally been performed by HPLC with UV, PDA, or MS detection. As it is essential to detect and measure all of the impurities in the sample, it is necessary to have a high resolution separation process. This usually involves long analysis times resulting in low throughput. As candidate pharmaceutical compounds become more potent and are dosed at lower and lower levels, ever more sensitive assays are needed to detect and measure impurities. The low throughput of HPLC can become the rate-limiting step in product release testing or process evaluation. Since much of the process of impurity identification involves the coupling of LC to sophisticated MS, any reduction in analysis time will result in a more efficient use of these significant investments. Analytical technology advances such as UPLC and UPC offer significant improvements in throughput and sensitivity, with benefits to the process of product release and identification of drug-related impurities. The most characteristic feature of the development in the methodology of pharmaceutical and biomedical analysis during the past 25 years is that HPLC became undoubtedly the most important analytical method for identification and quantification of drugs, either in their active pharmaceutical ingredient or in their formulations during the process of their discovery, development and manufacturing.
Unprecedented growth in popularity of complementary medicines raised concerns about their quality and safety. So, there is need to develop methods for their sensitive, specific and accurate analysis. Authenticity and similarity of medicinal nutrients and strict procedures of material handling are significant features to maintain the quality of herbal preparations. Genome based methods to authenticate these plants revolutionized the authentication process. Developing DNA molecular markers by sequencing a standard zone of the DNA is the best technique to identify the adulterants as well as to authenticate the required species of medicinal plants. Application of molecular biological technique serves as one of the very consistent system for authentication of natural herbal materials. The progress of authentic analytical methods is a major challenge to scientists as natural products are optimized as drug like molecules. This research work is based on the application of PCR technique for authentication of GlycyrrhizaglabraL. in its crude form as well as in final dosage form, i.e., Hamdard’sJoshanda, Marhaba’sJoshanda, GNC herbal supplement and JoshabaSadar (Chest tea) available in market, provided isolated DNA from dried roots of the sample was used as templates in PCR. All the products gavethe desired results except one. It proved to be a complementary tool to control quality of herbal materials alone and in different marketable herbal product having Glycyrrhiza spp. as their active ingredient.
New psychoactive substances are an emerging topic in analytical toxicology. They can lead to severe and even fatal intoxications whether intentionally or unintentionally consumed. To detect them in human biosamples, hyphenated mass spectrometry (MS) is currently the gold standard. We will highlight in this article the role of chromatography coupled MS in the field of NPS analysis and discuss prerequisites and recent developments.